Kinases/Enzyme Drug Discovery Assays and Products
Membrane protein stable cell lines are widely used in many areas of biomedical research. Creative Biolabs can offer membrane protein stable cell lines to stablish in vitro models for High Throughput Screening.
Creative Biolabs' membrane preparations are useful for membrane protein research. We offer membrane preparations to study the role of membrane proteins in diseases. Membrane preparations from Creative Biolabs are quality-assured frozen membranes from cells expressing recombinant or natural receptors.
Creative Biolabs offers high-quality, innovative tools to help research groups accelerate membrane protein drug discovery. They can be found by targets. If there is no product that meets your needs, please contact us.
538 protein kinases that transfer a γ-phosphate group from ATP to serine, threonine, or tyrosine residues are encoded in the human genome. Many of these kinases are linked to the development and spread of human cancer. Clinical therapy has been successful in the recent development of small-molecule kinase inhibitors for the treatment of various forms of cancer. In terms of pharmacological targets, protein kinases are second only to G-protein-coupled receptors in popularity. 37 protein kinase inhibitors have been approved by the FDA for the treatment of cancers like breast and lung cancer since the first one was created in the early 1980s. Additionally, several kinase-specific inhibitors exist in the preclinical stage of therapeutic research, and about 150 kinase-targeted medications are currently undergoing clinical phase studies.
Fig.1 Overview of signal transduction pathways.1
To meet the needs of our clients, Creative Biolabs can provide a wide variety of kinases/enzyme related assays and products:
Kinases move a phosphate moiety from a high-energy molecule (such as ATP) to their substrate molecule. Kinases function more quickly because the phosphoryl group and substrate are correctly positioned within their active regions. To evaluate your candidate's therapeutic effects on the targets, Creative Biolabs offers kinases/enzyme in vitro assays.
Most significant diseases, such as diabetes, neurological problems, or cancer, can be caused by the disruption, destruction, overexpression, or elimination of kinase genes. Additionally, it can result in cellular death, behavioral issues, metabolic problems, or behavioral problems. Kinase cell lines are available from Creative Biolabs for the research of membrane protein drugs.
Creative Biolabs offers quality-assured frozen membranes from cells expressing recombinant kinase proteins for facilitating the study of kinases.
Types of Kinase Inhibitors
Kinase inhibitors are particularly effective at treating cancer, especially when they target particular mutations that are primarily responsible for tumorigenesis. According to their ability to catalyze the transfer of the terminal ATP phosphate to the substrates, which often contain a serine, threonine, or tyrosine residue, they are divided into different categories. Types I, II, III, and IV kinase inhibitors were used to categorize small molecule protein kinase inhibitors. The type I kinase inhibitor is a small molecule that binds to a kinase's active conformation in the ATP pocket; the type II inhibitor is a small molecule that binds to a kinase's inactive conformation; the type III inhibitor is an allosteric or non-ATP competitive inhibitor; and the type IV inhibitor is a new class of kinase inhibitors that bind to protein kinases with the DFG-Asp in and C-helix out conformation.
Fig.2 Categorization of different kinases implicated in human cancer.2,3
References
- Image retrieved from https://commons.wikimedia.org/ wiki/File:Signal_transduction_ pathways.svg, Cybertory, 2010, used under CC BY 4.0, without any modification.
- Bhullar, Khushwant S., et al. "Kinase-targeted cancer therapies: progress, challenges and future directions." Molecular cancer 17 (2018): 1-20.
- Image retrieved from Figure 2 "Categorization of different kinases implicated in human cancer." Bhullar, et al. 2018, used under CC BY 4.0. The original image was modified by extracting and the title was changed to "Categorization of different kinases implicated in human cancer.".
