Virology Drug Discovery Assays and Products
Membrane protein stable cell lines are widely used in many areas of biomedical research. Creative Biolabs can offer membrane protein stable cell lines to stablish in vitro models for High Throughput Screening.
Creative Biolabs has developed a comprehensive list of membrane protein tools to support the development of antibody and other therapeutic. We are dedicated to bringing more value to our clients by providing stock products, more technical resources and custom protein production services.
Creative Biolabs offers high-quality, innovative tools to help research groups accelerate membrane protein drug discovery. They can be found by targets. If there is no product that meets your needs, please contact us.
The majority of the top 10 global health hazards, according to the World Health Organization (WHO), are viruses. The list also includes other high-threat pathogens such as COVID-19, Zika virus (ZIKV), Nipah virus, Middle Eastern Respiratory Syndrome coronavirus (MERS-CoV), Severe Acute Respiratory Syndrome coronavirus (SARS-CoV-1), and AIDS caused by the Human Immunodeficiency Virus (HIV), a global influenza pandemic, Dengue virus (DENV), and viral diseases caused by the Ebola.
Fig.1 Viruses entry cells.1,2
To meet the needs of our global customers, Creative Biolabs develops and provides high-quality virology related products:
The study of the structure, taxonomy, infection, and reproduction of viruses and virus-like substances, as well as their physiology and immune interactions with the host cell, is the main focus of virology, which aims to understand the symptoms that viruses and virus-like substances produce and how to treat them. For our global clients, Creative Biolabs provides virology cell lines.
While HTS aids in automating research and accelerating development time, membrane ligand binding assays enable us to observe how a ligand or novel synthetic chemical interacts with a target protein and evaluate the affinity and kinetics of those interactions. For the purpose of helping the research of virology, Creative Biolabs provides quality-assured frozen membranes from cells expressing recombinant or natural proteins.
Antiviral Drug Targets
A viral enzyme that is crucial for virus replication is the target of the majority of licensed antiviral medications. Drug targets for viral (DNA or RNA) polymerases have had great success. The two main groups of polymerase inhibitors are (i) nucleoside analogues and (ii) non-nucleoside allosteric inhibitors. Although they bind to the polymerase, allosteric inhibitors do not bind to the catalytic active site, which results in conformational changes that compromise polymerase activity. Examples include HIV non-nucleoside reverse transcriptase inhibitors (NNRTIs). The purine and pyrimidine derivatives known as nucleoside analogue inhibitors are chemically produced and have base or sugar components altered in a way that prevents the developing nucleotide chain during replication.
Approaches to Antiviral Drug Discovery
- Utilizing secure pseudoviruses is one solution. Pseudoviruses are chimeric particles created with the target pathogenic virus's envelope proteins displayed on the surface of a benign carrier virus. Pseudoviruses may employ the carrier virus's envelope proteins for binding and entrance, but they still maintain the native functionalities necessary to start the target virus' infection and viral replication, which are identified by the activity of a reporter gene. Another option for isolating replication inhibitors is to use viral mini-replicons that express reporter genes instead of infectious particles. These ex vivo techniques' sensitivity makes them especially helpful for identifying prospective antiviral candidates from natural product extracts before moving on to phenotypic screening with live virues.
- An alternate strategy is to examine the impact of substances on target viral proteins in vitro to see if they can obstruct important protein-protein interactions or inhibit enzymes like RdRps and proteases. Larger quantities of recombinant viral proteins can be produced without risk and employed in a number of tests with biophysical techniques like surface plasmon resonance. Given their biological relevance and inherent chemical and structural diversity, screening natural compounds for antiviral activity is particularly alluring. Utilizing a virtual screening strategy against numerous different viral proteins is one technique to include more natural compounds and/or derivatives in antiviral screening.
References
- Adamson, Catherine S., et al. "Antiviral drug discovery: preparing for the next pandemic." Chemical Society Reviews 50.6 (2021): 3647-3655.
- Distributed under Open Access License CC BY 3.0, without modification.
