Epigenetic Screening Services
Creative Biolabs provides the highest caliber epigenetic screening and profiling services. Our scientists, who are recognized authorities in this area, will offer you top-notch assistance with the identification of epigenetic drugs. We have developed the knowledge and experience necessary to progress and quicken your epigenetic target-based drug discovery initiatives.
Epigenetic modifications are variations in gene expression that are heritable and unrelated to a gene's nucleotide sequence. Four basic processes are involved in the epigenetic control of genes: (i) changes in the DNA methylation pattern; (ii) modifications to histones; (iii) nucleosome location and occupancy; and (iv) post-transcriptional modification through non-coding RNAs. Deviations from these regulatory pathways can result in compromised gene expression and the conversion of healthy cells into cancerous ones. These epigenetic changes frequently precede genetic changes, making them potentially useful as biomarkers for cancer screening, diagnosis, prognosis, and monitoring. The epigenetic screening services in Creative Biolabs include:
Cell-based Epigenetic Assay Services
Creative Biolabs offers Cell-based Epigenetic Assay Services to help the drug development.
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Bromodomain Assay Services
Creative Biolabs offers Bromodomain Assay Services to study epigenetic reader domains.
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Methyltransferase Assay Services
Creative Biolabs offers Methyltransferase Assay Services for compound screening and profiling.
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Demethylase Assay Services
Creative Biolabs offers Demethylase Assay Services for compound screening and profiling.
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Histone Acetyltransferase Assay Services
Creative Biolabs offers Histone Acetyltransferase Assay Services for HAT enzymes.
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Histone Deacetylase Assay Services
Creative Biolabs offers Histone Deacetylase Assay Services using fluorescence-based assays.
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Isocitrate Dehydrogenase Assay Services
Creative Biolabs offers Isocitrate Dehydrogenase Assay Services measuring enzyme activity in a coupled system.
Learn MoreCell-based Epigenetic Assay Services
Utilizing intact cells' physiological milieu to investigate novel therapeutic candidates is a useful application of cell-based epigenetic assays. Following the initial screening of novel compounds in biochemical assays, they are a reasonable next step since they provide important biologically relevant information regarding the compounds' efficacy.
Protein domains known as bromodomains are crucial for attracting a lot of protein and multiprotein complexes to lysine acetylation sites because they are able to identify acetylated lysine residues. They are well-liked targets for drug discovery and have a significant function in chromatin biology.
Methyltransferase Assay Services
Whereas arginines can only be mono- or dimethylated, lysines can be mono-, di-, or trimethylated. Monomethylation of both terminal guanidino nitrogens or dimethylation of one of the terminal guanidino nitrogens results in dimethylated arginine residues. Depending on the location and level of methylation, histones can have either an activating or repressive mark.
Demethylases are involved in the pathogenesis of several human disorders, most notably cancer. Many types of cancer have been linked to either arginine and lysine HMT dysregulation or mutations. For example, lysine methyltransferase G9a is dysregulated in mutations and cancers of the liver, prostate, and lung.
Histone Acetyltransferase Assay Services
An important class of epigenetic enzymes called histone acetyltransferases (HATs) is involved in transcriptional control, DNA damage repair, and nucleosome assembly. Numerous human illnesses, including cancer, are linked to HATs.
Histone Deacetylase Assay Services
Enzymes known as histone deacetylases (HDACs) remove acetyl groups from ɛ-N-acetyl lysine amino acids on both histones and non-histone proteins, including cytoplasmic and transcription factors. Condensed chromatin and inhibited gene transcription are the results of histone deacetylation.
Isocitrate Dehydrogenase Assay Services
Compound profiling and screening are well-suited for isoketone dehydrogenase tests. The metabolic enzymes IDH1 and IDH2 are essential for the conversion of isocitrate to 𝜶-ketoglutarate (𝜶KG) and the cofactor NADPH.
The disease's molecular heterogeneity as well as additional significant genes and pathways were discovered by epigenetic screening. Strong and independent prognostic and predictive screening markers that are able to reliably identify patient molecular heterogeneity as well as predict therapeutic response, resistance to particular treatments, and susceptibility to malignancies become essential. Creative Biolabs is committed to providing the best possible quality in epigenetics target screening and profiling services. Please contact us for more information.
