Membrane Protein Stable Cell Line Development Services
Creative Biolabs offers membrane protein stable cell line that can overexpress, knockdown, or knockout the target protein of interest to help our clients for membrane protein expression, membrane protein antibody discovery, membrane protein functional studies, membrane protein drug screening etc.
Membrane proteins play a key role in cellular function and therapeutic targets, and mediate processes that are critical for the flourishing of biological cells. Membrane-embedded transporters carry ions and bigger solutes across membranes, whereas receptors facilitate communication between the cell and its surroundings and membrane-embedded enzymes catalyze chemical reactions. The difficulty in preparing a sufficient amount of these membrane proteins in order to develop a functional antibody and conduct functional assays is one of the most significant challenges.
Membrane Protein Overexpression Cell Line Development
Creative Biolabs offers membrane protein cell line development service to help you stably express your gene of interest constitutively or inducibly.
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Membrane Protein Knockdown Cell Line Development
Creative Biolabs offers different approaches to knockdown the membrane protein of interest, including knockdown with siRNA and knockdown with shRNA.
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Membrane Protein Cell Line Development with CRISPR/Cas9
Creative Biolabs has extensive experience in the field of membrane protein cell line development. Our scientists have developed several perfect membrane protein knockout cell lines with CRISPR/Cas9 technology.
Learn MoreMembrane Protein Overexpression Cell Line Development
Overexpression of a membrane protein may cause deleterious impacts on cell development, resulting in low expression or even deletion. Hence, deciding whether to use a constitutive or inducible promoter to express the membrane protein of interest is crucial. Creative Biolabs offers membrane protein cell line development service to help you stably express your gene of interest constitutively or inducible.
Fig.1. Strong and weak promoters for constitutive expression.
Membrane Protein Knockdown Cell Line Development
A major approach in the field of mammalian cell biology is the manipulation of the expression of genes of interest in selected cell lines, with the aim to reveal one or several of the gene's function(s) using transient/stable overexpression or knockdown of the gene of interest. The simplest method for RNA interference is the cytosolic delivery of siRNA oligonucleotides, this technique is limited to cells capable of transfection and is primarily utilized during transient in vitro studies. The introduction of shRNA into mammalian cells through infection with viral vectors allows for stable integration of shRNA and long-term knockdown of the targeted gene. Creative Biolabs offers different approaches to knockdown the membrane protein of interest.
Fig.2. Structure of siRNA and shRNA. (A) siRNAs. (B) shRNAs.
Membrane Protein Cell Line Development with CRISPR/Cas9
Gene knockout (KO) is an important technique for determining the roles of both coding and non-coding genomic regions. CRISPR-associated protein 9 (CRISPR/Cas9), a system of clustered regularly interspaced short palindromic repeats, has emerged as one of the most interesting gene knock-out techniques in recent years. The CRISPR/Cas9 system relies simply on base-pairing between the single-guide RNA (sgRNA) and the target DNA. With a minimal requirement in target design and straightforward construction of sgRNAs, it facilitates widespread application in either fundamental research. Creative Biolabs offers CRISPR/Cas9 system to edit the membrane protein of interest.
Fig.3. CRISPR/Cas9 system.
Creative Biolabs has extensive experience in the field of membrane protein stable cell line development. Our scientists have developed several membrane protein stable cell lines. We are pleased to provide custom membrane protein cell line to meet our clients’ individual requirement. Please feel free to contact us for more information.
