Membrane Staining and Imaging Reagents
Creative Biolabs offers numerous membrane staining and imaging reagents which are created for simple labeling of live cells, fixed cells, or live cells as well as for fixation and subsequent immunostaining.
The majority of the tasks of these membranes are performed by membrane proteins, which are proteins that are a part of or interact with cell membranes. About one-third of human proteins are membrane proteins, which are in charge of regulating activities essential to biological cells' survival. Membrane proteins are found in many locations throughout the membrane and have a variety of structural variations. They perform a wide range of tasks, and the quantity and variety of proteins found on a given membrane might change.
In multi-color fluorescence imaging, researchers commonly want to mark the cell surface or plasma membrane to see cellular borders or cell morphology. Numerous membrane staining and imaging reagents are available from Creative Biolabs and are created for simple labeling of live cells, fixed cells, or live cells as well as for fixation and subsequent immunostaining.
Click on any link below for further information about our membrane staining and imaging reagents.
Membrane Dyes and Reagents
Lipophilic fluorescent dyes fall within the category of cell membrane fluorescent probes. They are used to mark hydrophobic tissues and cell membranes. They are dissolved in DMF, DMSO, and methanol and kept at -20°C in a dry, dark environment. They are polarization dependent, have a high quenching coefficient, and have a very limited excitation lifespan. As environmentally sensitive fluorescent dyes, cell membrane fluorescent probes' fluorescence intensity is markedly increased when they are attached to membranes or to lipophilic substances. When they reach the correct concentration, they uniformly stain the entire cell after gradually diffusing through the intracellular plasma membrane after entering the cell. Ethanol can be used to make the stock solution of cell membrane fluorescent probes, and they have no negative effects on the viability of cells. After cell membrane fluorescent probe staining, paraformaldehyde can be fixed, although permeabilization after staining is not advised. Creative Biolabs offers membrane dyes and reagents for our global customers.
Probe and Stain Indicators
Probe and stain indicators interact with their surroundings and undergo changes that alter their fluorescent characteristics. Because the detection limits for fluorescence are lower than those for colorimetric light absorption—by a factor of 102–104—greater sensitivity can be attained by utilizing fluorescent compounds. Planar, rigid, unsaturated organic compounds that fluoresce, like polycyclic aromatics, are frequently conjugated. Such compounds will form complexes if the surrounding groups can coordinate with the metal centers. Creative Biolabs provides a series of probe and stain indicators to meet customer needs.
Genetically Encoded Sensors
Optical reporting, genetic encoding, or both can result in a number of compelling advantages for genetically encoded sensors. Reporters can be built from proteins that react to a range of neural stimuli thanks to genetic encoding. In order to obtain data that electrodes and functional magnetic resonance imaging cannot, optical reporters have been developed for vesicle release, changes in neurotransmitter concentrations, transmembrane voltage, and intracellular calcium dynamics. Creative Biolabs can offer genetically encoded sensors related products to contribute to the success of drug discovery.
