GPCR Cell Lines
Membrane protein stable cell lines are widely used in many areas of biomedical research. Creative Biolabs can offer membrane protein stable cell lines to stablish in vitro models for High Throughput Screening.
Creative Biolabs offers high-quality, innovative tools to help research groups accelerate membrane protein drug discovery. They can be found by targets. If there is no product that meets your needs, please contact us.
Background of GPCR Cell Lines
Cell lines have been broadly used in the studies of physiological and pathological processes of particular cells. G protein-coupled receptors are the largest family of seven transmembrane protein receptors on the cell surface. They couple to the G proteins by the activation of various ligands, regulating multiple cellular signaling pathways, which participate in diverse pathological and physiological processes. GPCRs have been used as drug targets for considerably large numbers of therapeutics. The development of stable GPCR cell lines enables researchers efficiently to study GPCRs.
Application of GPCR Cell Lines
GPCR cell lines are artificially engineered to stably express GPCRs so that the researchers could more comprehensively discover the mechanisms and functions of GPCRs as well as novel GPCR-related drug therapies. Through the measurement of GPCR proteins could investigate GPCR signaling pathways, screen the agonists and antagonists of GPCRs, and profile compounds. The GPCR cell lines enable the detection of GPCRs through the measurement of second messengers, such as the concentration of cAMP or Ca2+, ligands binding triggered-arrestin recruitment, and GPCR internalization. The measurements could be readout through fluorescence or chemiluminescence. Therefore, GPCR cell-based assays play a crucial role in the GPCR-related drug discovery process, including target validation, high throughput screening, drug design, etc.
