Ion Channel Cell Lines
Membrane protein stable cell lines are widely used in many areas of biomedical research. Creative Biolabs can offer membrane protein stable cell lines to stablish in vitro models for High Throughput Screening.
Creative Biolabs offers high-quality, innovative tools to help research groups accelerate membrane protein drug discovery. They can be found by targets. If there is no product that meets your needs, please contact us.
Background of Ion Channel Cell Lines
Cell-based assays are indispensable in drug development. Stable cell lines could assist researchers more efficiently and comprehensively to explore the physiology, pathology, and differentiation of specific cells for further therapeutic discovery. Ion channels are a family of integral and pore-forming membrane proteins expressed on almost all cells. Ion channels are capable of generating electrical signals when an ion moves in or out through the ion channel, which could be detected for the research. Ion channels participate in multiple pathological and physiological processes. The disfunction of ion channels could cause several corresponding genetic and autoimmune diseases and cancers, such as episodic ataxia, familial hemiplegic migraine, Long QT syndrome, glioblastoma, etc. Therefore, the ion channels become prospective drug targets.
Fig.1 Ion channels. (Kabra, 2020)
Application of Ion Channel Cell Lines
Ion channel blockers and activators have been discovered and used as therapeutics. Ion channel blockers are able to downregulate the ion channel activity and conductance, such as sodium channel blocker saxitoxin and potassium channel blocker dendrotoxin. Ion channel activators are capable of promoting the opening or activation of ion channels, including potassium channel activators minoxidil and sodium channel activators DDT, etc. Stable ion channel cell lines enable researchers to discover ion channels, screen compounds, and discover drugs in an early stage. With advanced technologies applied to the detection of ion channel electrical signals, including manual or automated electrophysiology, fluorescence, and RT-PCR, we can more accurately study the mechanisms and functions of ion channels and identify the ion channel targeting drug candidates.
Published Data
| Paper Title | TRPC6 channels are required for proliferation, migration and invasion of breast cancer cell lines by modulation of Orai1 and Orai3 surface exposure |
| Journal | Cancer |
| Published | 2018 |
| Abstract | Transient receptor potential channels primarily function by causing variations in the cytosolic Ca2+ concentration to transmit signaling information from various stimuli to a range of cellular activities. TRPC, TRPM, and TRPV subfamilies are three types of ion channels, playing a role in tumorigenesis, especially breast cancer cell proliferation, migration, and invasion. The study aimed to investigate the mechanism for the modulation of Ca2+ influx and the development of different cancer hallmarks in breast cancer cells. |
| Result |
The results demonstrated that the estrogen receptor positive and triple negative breast cancer cell lines, including both MCF7 and MDA-MB-231 cell lines, exhibit enhanced expression of the TRPC6 channel as compared to the non-tumoral MCF10A cell line. The BrdU incorporation assay and wound healing assay, and Boyden chamber assay were performed. The result showed that in vitro TRPC6 knockdown using shRNA impaired MCF7 and MDA-MB-231 cell proliferation, migration, and invasion. In addition, they found that TRPC6 played a relevant role in the activation of store-operated Ca2+ entry in the breast cancer cell lines using RNAi-mediated TRPC6 silencing and overexpression of the pore-dead dominant-negative TRPC6 mutant. Furthermore, the results indicated that TRPC6 interacted with Orai1 and Orai3 in MCF7 and MDA-MB-231 cells.
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Fig.2 TRPC6 expression is required for MCF7 and MDA-MB-231 cell proliferation. (Jardin, 2018)
References
- Kabra, M.; Pattnaik, B. R. Sensing through Non-Sensing Ocular Ion Channels. International Journal of Molecular Sciences. 2020, 21(18): 6925.
- Jardin, I.; et al. TRPC6 channels are required for proliferation, migration and invasion of breast cancer cell lines by modulation of Orai1 and Orai3 surface exposure. Cancers. 2018; 10(9): 331.
