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  • mProX™ Human S1PR1 Stable Cell Line

    [CAT#: S01YF-0923-PY102]
    Product Category:
    Membrane Protein Stable Cell Lines
    Subcategory:
    GPCR Cell Lines

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    Based on this stable cell line, we also provide cell-based in vitro assays to evaluate the effects of your compounds or antibodies.

    Sub Cat Product Name Target Protein Species Host Cell Type Assay Types Inquiry Datasheet
    S01YF-1122-KX576 Magic™ Rat S1PR1 in Vitro Calcium Flux Assay Rat CHO-K1-Gqi5 Calcium Flux Assay
    S01YF-1122-KX577 Magic™ Rat S1PR1 in Vitro cAMP Assay Rat CHO-K1 cAMP Assay

    Product Information

    Target Protein
    S1PR1
    Target Family
    Lysophospholipid Family
    Target Protein Species
    Human
    Host Cell Type
    Huh7;SK-Hep1;CHO-K1;HEK293
    Target Classification
    GPCR Cell Lines
    Target Research Area
    Immunology Research;CNS Research
    Related Diseases
    Dermatitis, Atopic, 7;Second-Degree Atrioventricular Block
    Gene ID
    Human: 1901
    UniProt ID
    Human: P21453

    Product Properties

    Biosafety Level
    Level 1
    Activity
    Yes
    Quantity
    10⁶ cells per vial
    Applications
    Sphingosine-1-phosphate receptor 1 (S1PR1) is a central figure in scientific explorations, particularly in the context of vascular biology and immunology. It has been found to play a role in hepatocellular carcinoma progression by influencing metabolic reprogramming of ceramide in vascular endothelial cells. Additionally, its involvement in modulating the allogeneic response of T cells, impacting graft-versus-host disease, has been highlighted. The potential of S1PR1 as a therapeutic target is evident, with agonists and antagonists being developed to modulate its activity in various diseases.

    Protocols

    Please visit our protocols page.

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    FAQ

    chat John (Verified Customer)

    How does S1PR1 signaling contribute to the survival of retinal ganglion cells in experimental glaucoma? Jan 08 2023

    chat Patrick Liam (Creative Biolabs Scientific Support)

    S1PR1 signaling plays a vital role in the survival of retinal ganglion cells and neurons in the dorsolateral geniculate nucleus in experimental glaucoma. Siponimod, an S1PR1 modulator, exerts direct neuroprotective effects, indicating a potential therapeutic application for glaucoma patients. Jan 08 2023

    chat Nancy (Verified Customer)

    What is the role of S1PR1 in the regulation of IL-1β expression during NDV infection? Jul 18 2021

    chat Patrick Liam (Creative Biolabs Scientific Support)

    NDV infection promotes S1PR1 expression and induces IL-1β expression through p38, JNK/MAPK, and NLRP3/caspase-1 inflammasomes. S1PR1 regulates IL-1β expression mainly through the NLRP3/caspase-1 inflammasome, suggesting a potential target for modulating immune responses during viral infections. Jul 18 2021

    Published Data

    Fig.1 Elevating S1PR1 levels within HAECs enhances the proliferation potential of hepatocellular carcinoma (HCC) cells.

    CCK-8 experiments were conducted to assess the viability of hepatocellular carcinoma (HCC) cell lines (Huh7 and SK-Hep1) when co-cultured with endothelial cells (ECs) exhibiting S1PR1 gene knockdown.

    Ref: Wang, Xuehong, et al. "S1PR1 induces metabolic reprogramming of ceramide in vascular endothelial cells, affecting hepatocellular carcinoma angiogenesis and progression." Cell Death & Disease 13.9 (2022): 768.

    Pubmed: 36068200

    DOI: 10.1038/s41419-022-05210-z

    Research Highlights

    Wunsch F, et al. "Structural determinants of sphingosine-1-phosphate receptor selectivity.." Archiv der Pharmazie, 2023.
    In this study, the researchers focused on the drug fingolimod, which is the prodrug of fingolimod-1-phosphate (F1P) that was the first approved sphingosine-1-phosphate receptor (S1PR) modulator for treating multiple sclerosis. F1P targets all five S1PR subtypes, including S1PR(1) and S1PR(3). While S1PR(1) activation is linked to immune modulatory effects, S1PR(3) activation can cause cardiac adverse effects. To address this, the researchers used a combination of molecular dynamics simulations and three-dimensional pharmacophores (dynophores) to investigate the binding site characteristics of specific S1PR subtypes. This research has potential implications for the development of new drugs targeting S1PR subtypes, such as ozanimod and siponimod, as well as pharmaceutical tool compounds like CYM5541.
    Pubmed: 37806764   DOI: 10.1002/ardp.202300387

    Skoug C, et al. "Density of Sphingosine-1-Phosphate Receptors Is Altered in Cortical ." Neurochemical research, 2023.
    Sphingosine-1-phosphate (S1P), a phosphosphingolipid, has diverse biological functions. It acts as an intracellular second messenger and as an extracellular ligand to five G-protein coupled receptors (S1PR1-5). In the brain, S1P plays a crucial role in regulating neuronal proliferation, apoptosis, synaptic activity, and neuroglia activation. Recently, altered S1P metabolism has been observed in neurodegenerative disorders. Previous research has demonstrated the presence of S1PRs in nerve terminals, exhibiting distinct sub-synaptic localization and neuromodulation actions. Given that synaptic dysfunction is associated with conditions such as type 2 diabetes (T2D), the researchers hypothesized that S1P signaling may be modified in nerve terminals. The current study aimed to determine the density of S1PRs in cortical synaptosomes in insulin-resistant Goto-Kakizaki (GK) rats and Wistar control rats, as well as in mice fed a high-fat diet (HFD) and low-fat-fed controls. The results revealed that, compared to their respective control groups, GK rats had similar cortical S1P levels despite having higher plasma levels. However, they had a lower density of S1PR1, S1PR2, and S1PR4 in nerve-terminal-enriched membranes. Similarly, HFD-fed mice showed increased plasma and cortical S1P concentrations and a decreased density of S1PR1 and S1PR4. These findings suggest that there may be altered S1P signaling in synapses of models with insulin resistance and diet-induced obesity, potentially implicating S1P signaling in T2D-associated synaptic dysfunction.
    Pubmed: 37794263   DOI: 10.1007/s11064-023-04033-4

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR CLINICAL PROCEDURES" For licensing inquiries, please contact
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