mProX™ Human EDNRA Stable Cell Line

He X, et al. "Assessment of the anti-inflammatory mechanism of quercetin 3,7-dirhamnoside using ." Chemical biology & drug design, 2023.
Pouzolzia zeylanica (L.) Benn. is a popular Chinese herbal medicine known for its anti-inflammatory and pus-removal properties. To explore its potential anti-inflammatory mechanism, the main flavonoid component, quercetin 3,7-dirhamnoside (QDR), was extracted and purified. Through network analysis, it was predicted that QDR targets a variety of proteins involved in inflammation. Molecular docking, dynamics simulations, and in vitro experiments confirmed 342 potential QDR targets. Among these, RAC1, AKT1, NOS3, mTOR, EGFR, GRB2, and EDNRA were identified as potential targets, regulating immune response, apoptosis, and anti-inflammatory activity through pathways such as PI3K/AKT, cAMP, and Ras signaling. Additionally, molecular docking and dynamics simulations revealed strong binding abilities between QDR and AKT1, mTOR, and NOS3. In RAW264.7 macrophages, QDR effectively inhibited the expression of inducible nitric oxide synthase and pro-inflammatory cytokines induced by lipopolysaccharides, while upregulating the expression of NOS3 and regulating the levels of AKT1 and mTOR. This study provides insight into the potential anti-inflammatory mechanism of QDR and its main flavonoid compound, highlighting its potential as a therapeutic agent for inflammatory conditions.
Pubmed: 37806949   DOI: 10.1111/cbdd.14346

Ling W, et al. "EDNRA-Expressing Mesenchymal Cells Are Expanded in Myeloma Interstitial Bone ." Cancers, 2023.
Multiple myeloma (MM) has been shown to cause dysfunction in bone marrow (BM) mesenchymal cells and promote the growth of new blood vessels. In previous studies, it was observed that pericytes and smooth muscle cells (SMCs) detached from vessels and transformed into cancer-associated fibroblasts. In this study, the researchers focused on the role of the pericyte and SMC marker, endothelin receptor type A (EDNRA), in MM progression. They found that EDNRA expression gradually increased as the disease progressed, with high-risk MM patients showing higher levels of expression compared to low-risk patients. Furthermore, EDNRA expression was highest in focal lesions. Analysis of unexpanded BM mesenchymal cells revealed that a subset of cells with high expression of proliferation genes also expressed EDNRA. Immunohistochemistry studies showed that the number of EDNRA+ cells in the interstitial BM increased as MM progressed and these cells were mostly found in areas near the MM focal growth. These EDNRA+ cells were detached from CD34+ angiogenic cells and coexpressed the pericyte markers RGS5 and periostin. This suggests that they may have originated from pericytes or SMCs that detached from vessels. These findings introduce EDNRA as a novel microenvironmental biomarker in MM and indicate that the presence of detached EDNRA+ cells is associated with disrupted vasculature and increased angiogenesis.
Pubmed: 37760488   DOI: 10.3390/cancers15184519