mProX™ Human P2RY12 Stable Cell Line

Product Information

Target Protein

P2RY12

Target Family

P2Y Family

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Cardiovascular Research

Related Diseases

Bleeding Disorder; Nizon-Isidor Syndrome

Gene ID

64805

UniProt ID

Q9H244

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

In order to distinguish healthy mouse CNS microglial cells from other cell types such as tissue-resident macrophages or blood-derived monocytes, the purinergic receptor P2Y12 (P2RY12) was proposed as a unique marker for these cells. A unique marker for microglial cells in human brains, P2RY12, has been proposed. P2RY12 is a member of the P2 purinergic receptor family, which also includes seven other transmembrane G protein coupled receptors (GPCRs) involved in ATP- and ADP-mediated in vitro cell motility. In addition to microglial cells, active platelets also express P2RY12. In P2RY12 knockdown animals, microglial chemotaxis and the expansion of microglial foot processes in response to CNS damage were severely impaired. The customized P2RY12 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Ava

The P2RY12 Stable Cell Line is great for cardiovascular research. Dec 24 2022

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chat Ethan

Flow cytometry is used to verify the surface expression of the P2RY12 protein, and qPCR is used to verify the engineering results at the mRNA level. Jul 19 2022

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FAQ

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Published Data

Fig.1 Laser injury does not influence capillary perfusion; instead, it causes an accumulation of juxtavascular microglia processes.

Focused laser injury location in the cerebral cortex of CX3CR1+/EGFP rats was analyzed using immunohistochemistry. P2RY12 and E-cadherin, which are both strongly expressed in the microglial cell processes around the damage site, colocalized with EGFP (green). Occludin (red), in contrast, was exclusively found in vascular endothelial cells and not in microglial cells.

Ref: Lou, Nanhong, et al. "Purinergic receptor P2RY12-dependent microglial closure of the injured blood-brain barrier." Proceedings of the National Academy of Sciences 113.4 (2016): 1074-1079.

Pubmed: 26755608

DOI: 10.1073/pnas.1520398113

Research Highlights

Immune brain cells called microglia play a role in neuroinflammation. Numerous proteins, including receptors that can be activated by mediators secreted in the microglial milieu, are expressed on their surface. Purinergic receptor expression could change in relation to the activation level of microglia among these receptors.
Gómez Morillas, Albert, Valérie C. Besson, and Dominique Lerouet. "Microglia and neuroinflammation: what place for P2RY12?." International journal of molecular sciences 22.4 (2021): 1636.
Pubmed: 33561958 DOI: 10.3390/ijms22041636

Following cerebrovascular injury, microglial cells have a hitherto unappreciated protective role in maintaining BBB integrity. Clopidogrel is frequently prescribed to individuals with coronary artery and cerebrovascular disease because it inhibits the platelet P2Y12 receptor.
Lou, Nanhong, et al. "Purinergic receptor P2RY12-dependent microglial closure of the injured blood-brain barrier." Proceedings of the National Academy of Sciences 113.4 (2016): 1074-1079.
Pubmed: 26755608 DOI: 10.1073/pnas.1520398113