mProX™ Human NPFFR1 Stable Cell Line

Zegeye T, et al. "Genome-wide diversity and admixture of five indigenous cattle populations from ." Frontiers in genetics, 2023.
The region of Tigray in Ethiopia is home to approximately eight per cent of the indigenous cattle population of the country. It is known as the historical center of Ethiopia and has been in contact with civilizations from the Fertile Crescent and the Indian subcontinent since ancient times. A study using whole genome sequencing was conducted on five cattle populations (Abergelle, Arado, Begait, Erob, and Raya) indigenous to this region. The study detected millions of novel genetic variations and identified important genes related to morphology, milk yield, meat quality, feed efficiency, immune response, heat resistance, and adaptation to harsh environments. The Tigray indigenous cattle show high genetic diversity and a low level of European taurine introgression, instead sharing a common ancestry with Asian indicine and African taurine cattle. The findings suggest potential for breeding improvement programs and contribute to the understanding of domestic cattle genetic diversity.
Pubmed: 37600659   DOI: 10.3389/fgene.2023.1050365

Aquino NSS, et al. "RFamide-related Peptide 3 Signaling via Neuropeptide FF Receptor Stimulates ." Endocrinology, 2023.
The family of RF-amide peptides, including kisspeptin (Kp) and RFamide-related peptide 3 (RFRP-3), regulates various physiological processes through binding to specific receptors. In this study, the role of the neuropeptide FF receptor 1 (Npffr1) in the control of prolactin (PRL) secretion by Kp and RFRP-3 was investigated. Results from ICV injections in ovariectomized, estradiol-treated rats revealed that Kp stimulates PRL and LH secretion, while RFRP-3 only affects PRL secretion. The Npffr1 antagonist RF9 and the selective antagonist GJ14 were both able to prevent the effects of Kp and RFRP-3 on PRL secretion, respectively. Furthermore, GJ14 was found to blunt the estradiol-induced PRL surge and amplify the LH surge in female rats. However, whole-cell patch clamp recordings showed no direct effect of RFRP-3 on the electrical activity of tuberoinfundibular dopaminergic (TIDA) neurons. These results suggest that RFRP-3 binds to Npffr1 to stimulate PRL release, which plays a role in the estradiol-induced PRL surge. This effect of RFRP-3 does not seem to involve a reduction in TIDA neuronal inhibition, but may involve the activation of a hypothalamic PRL-releasing factor.
Pubmed: 37403228   DOI: 10.1210/endocr/bqad102