mProX™ Human CFTR Stable Cell Line

Product Information

Target Protein

CFTR

Target Family

Cystic Fibrosis Transmembrane Conductance Regulator

Target Protein Species

Human

Host Cell Type

A2780; SKOV3; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Reproductive Research

Related Diseases

Cystic Fibrosis; Vas Deferens, Congenital Bilateral Aplasia Of

Gene ID

1080

UniProt ID

Q20BH0

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Instructions for producing the CF transmembrane conductance regulator (CFTR) protein are found in the CFTR gene. This protein serves as a conduit across the membrane of the cells that secrete digestive enzymes, perspiration, saliva, and mucus. Chloride ions, which are negatively charged particles, are transported into and out of cells via the channel. Chloride ion transport aids in regulating tissue water flow, which is essential for the synthesis of thin, fluid mucus. Other channels, such those that move positively charged particles called sodium ions across cell membranes, are also controlled by the CFTR protein. Organs like the pancreas and lungs cannot function normally without these channels. The customized CFTR stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat William

I am truly impressed with the performance of this CFTR cell line and the professionalism of Creative Biolabs. I highly recommend it to anyone. May 06 2020

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chat John

I purchased the CFTR cell line from Creative Biolabs. The provided documentation was comprehensive and helped me optimize my assay conditions. Nov 15 2020

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 The CFTR-specific shRNA expression pGFP-V-RS vectors were used to transfect the human A2780 and SKOV3 epithelial ovarian cancer cell lines, and the stably transfected cells were then chosen.

Western blot analysis was used to quantify the CFTR protein (~170 kDa) in each puromycin-resistant cell, GFP-positive cell colonies, and negative control cells. As an internal control, β-actin (~43 kDa) was employed.

Ref: Xu, Jiao, et al. "High level of CFTR expression is associated with tumor aggression and knockdown of CFTR suppresses proliferation of ovarian cancer in vitro and in vivo." Oncology Reports 33.5 (2015): 2227-2234.

Pubmed: 25738998

DOI: 10.3892/or.2015.3829

Research Highlights

Although it is a member of the ATP binding cassette (ABC) transporter superfamily, the cystic fibrosis transmembrane conductance regulator (CFTR) serves as an anion channel that is essential for the passage of salt and water through epithelial cells.
Csanády, László, Paola Vergani, and David C. Gadsby. "Structure, gating, and regulation of the CFTR anion channel." Physiological reviews 99.1 (2019): 707-738.
Pubmed: 30516439 DOI: 10.1152/physrev.00007.2018

Modulator for CFTR theratyping is a new and quickly developing discipline that may be able to find uncommon CFTR variations that respond to medicines that are either approved or under development.
Clancy, John Paul, et al. "CFTR modulator theratyping: Current status, gaps and future directions." Journal of Cystic Fibrosis 18.1 (2019): 22-34.
Pubmed: 29934203 DOI: 10.1016/j.jcf.2018.05.004