mProX™ Human CCR6 Stable Cell Line

Product Information

Target Protein

CCR6

Target Family

Chemokine Family

Target Protein Species

Human

Host Cell Type

HUVECs;CHO-K1;HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Immunology Research

Related Diseases

Limited Scleroderma;Autoimmune Disease Of Musculoskeletal System

Gene ID

Human: 1235

UniProt ID

Human: P51684

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

CCR6, known as the C-C chemokine receptor type 6, is another protein that has garnered attention in the scientific community. This receptor is known for its interaction with the ligand CCL20, forming the CCL20-CCR6 axis, which has been implicated in various pathological processes. For instance, the CCL20-CCR6 axis has been identified as playing a pivotal role in the progression of diseases like psoriasis and psoriatic arthritis. Moreover, research has highlighted the significance of the CCL20-CCR6 axis in endometriosis, where it mediates macrophages to promote the proliferation and migration of endometrial stromal cells by blocking autophagic flux. Additionally, the CCL20-CCR6 axis has been associated with cancer progression, emphasizing its potential as a therapeutic target. In the context of COVID-19, enrichment of CCR6+CD8+ T cells and CCL20 has been observed in the lungs of mechanically ventilated patients, suggesting a potential role in the disease's pathogenesis. Overall, the application of CCR6 in scientific research provides valuable insights into various physiological and pathological processes, from autoimmune diseases to cancer progression.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Cynthia (Verified Customer)

Is CCR6 only involved in inflammatory responses? Jun 24 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

No, while CCR6 is involved in inflammatory responses, it also plays a role in the migration of dendritic cells and the development of secondary lymphoid organs. Jun 24 2023

chat Ashley (Verified Customer)

Can CCR6 be considered a marker for certain diseases? Jan 17 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

Yes, increased expression of CCR6 has been associated with diseases like rheumatoid arthritis and other autoimmune diseases. Jan 17 2022

Published Data

Fig.1 Suppression of CCR6 in HUVECs partially counteracts the angiogenic impact of heightened DEPDC1 expression.

Tumor cell-conditioned medium (TCM) was harvested from Li-7 and Hep3B cells following treatment with pcDNA3.1 or DEPDC1 expression vectors. Employing HUVECs pre-exposed to either NC or CCR6 siRNA, a tube formation assay was conducted using the respective conditioned media. Key abbreviations encompass CCR6 (chemokine (C-C motif) receptor 6), HUVECs (human umbilical vein endothelial cells), DEPDC1 (DEP domain containing 1), TCM (tumor cell-conditioned medium), NC (negative control), and siRNA (small interfering RNA).

Ref: Guo, Wubin, et al. "DEPDC1 drives hepatocellular carcinoma cell proliferation, invasion and angiogenesis by regulating the CCL20/CCR6 signaling pathway." Oncology Reports 42.3 (2019): 1075-1089.

Pubmed: 31322256

DOI: 10.3892/or.2019.7221

Research Highlights

Zacharias ZR, Houtman JCD. "OMIP-099: 31-color spectral flow cytometry panel to investigate the steady-state ." Cytometry. Part A : the journal of the International Society for Analytical , 2023.
The authors have developed a 31-color panel for identifying the steady-state phenotype of T cells in human peripheral blood (Table 1). This panel was optimized using cryopreserved peripheral blood mononuclear cells (PBMC). The markers included in the panel were selected to characterize the steady-state phenotype of T cells, including CD45RA, CD45RO, CCR7, and CD95 to differentiate CD4, CD8, and gammadelta T cell subsets such as naive, T(EM), T(CM), T(EMRA), and T(SCM). Additionally, the panel includes markers for determining differentiation status (CD27, CD28), activation/antigen experience status (CD11a, CD49d, CD38, HLA-DR, CD56, and CD39), co-inhibitory marker expression (PD-1, TIM-3), and CD4 T helper subsets (CXCR3, CXCR5, CCR4, CCR6, Foxp3, CD25, and CD127). Overall, this optimized panel provides a comprehensive assessment of the steady-state phenotype of human T cells.
Pubmed: 37814476 DOI: 10.1002/cyto.a.24799

Akhtar M, et al. "T helper cell responses in adult diarrheal patients following natural infection ." Frontiers in immunology, 2023.
Infection with enterotoxigenic Escherichia coli (ETEC) results in the production of IgA antibodies against the heat labile toxin (LT) and colonization factors (CFs), which work together to protect against ETEC diarrhea. It has been suggested that the development of ETEC-specific long lived plasma cells and memory B cells requires the support of T helper (Th) cells. Therefore, the researchers aimed to investigate if individuals with natural ETEC diarrhea experience ETEC-specific Th cell responses and the relationship between these responses and IgA production. The study included hospitalized adults from Bangladesh who were diagnosed with ETEC diarrhea, as well as healthy individuals. By analyzing peripheral blood mononuclear cells and utilizing flow cytometry and ELISA, the research team found that ETEC patients had increased levels of memory Th cells with a CCR6+CXCR3- phenotype and mounted significant IgA responses against LT and CFs. They also observed a correlation between Th17-type responses and specific IgA production. These findings provide important insight into the potential role of Th17 cells in the development of protective immunity against ETEC infection and have implications for the evaluation of ETEC vaccine candidates.
Pubmed: 37809062 DOI: 10.3389/fimmu.2023.1220130