mProX™ Human ADRB3 Stable Cell Line

Desevin K, et al. "Adrenergic Reprogramming of Preexisting Adipogenic Trajectories Steer Naive Mural ." bioRxiv : the preprint server for biology, 2023.
In adult white adipose tissue, thermogenic beige adipocytes are promoted by cold or beta3-adrenoceptor activation. According to a comprehensive single-cell analysis, these cells originate from the reprogramming of current adipogenic pathways rather than from a single precursor. The main source of these pathways is the vascular mural progenitor cells expressing SM22. The crucial step in this process is the activation of Adrb3 in mature adipocytes, leading to the subsequent increase of Adrb1 in progenitor cells. Under thermoneutral conditions, the activation of both Adrb3 and Adrb1 together mimics the recruitment of SM22+ cells induced by cold. Lipolysis-derived eicosanoids, namely docosahexaenoic acid (DHA) and arachidonic acid (AA), trigger these pathways and can replicate the priming of progenitor cells in vitro. Overall, these findings present a strong model for the development of beige adipocytes in response to cold, highlighting the important relationship between mature adipocytes and mural cells during this process and showcasing the metabolic potential of this distinct cellular reservoir.
Pubmed: 37662295   DOI: 10.1101/2023.08.26.554950

Potocka N, et al. "Effects of the Trp64Arg Polymorphism in the ADRB3 Gene on Body Composition, ." Genes, 2023.
In this study of young, healthy adults (N=304), the authors aimed to examine the association of the ADRB3 rs4994 polymorphism with body composition, somatotype, cardiorespiratory fitness, and physical activity. The ADRB3 gene plays a role in energy expenditure through lipolysis. While previous studies have investigated the polymorphism in athletes, overweight individuals, and patients with obesity and diabetes, there has been no such research on young, healthy adults. Using a PCR-RFLP method, the researchers genotyped for the ADRB3 gene and assessed anthropometric measurements, somatotypes, cardiorespiratory fitness, and physical activity levels. In the male subgroup, the Trp64Trp genotype was associated with various body components such as waist and hip circumference, body fat percentage and mass, BMI, and ratios of waist circumference to height and hip circumference to height. The Trp64Trp genotype also showed an association with somatotype components. Conversely, the Arg allele was linked with ectomorphic components. In addition, the Trp64Trp genotype was positively correlated with maximal oxygen uptake and oxygen pulse. In the female subgroup, the Trp64Trp genotype was negatively associated with reported moderate-intensity exercise. Overall, the Trp64 allele was found to be related to anthropometric traits, somatotype, and physical performance parameters in males, while the Trp64Arg polymorphism was associated with the level of physical activity for moderate-intensity exercise in females.
Pubmed: 37628593   DOI: 10.3390/genes14081541