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  • Magic™ Human ADRB3 Lentivirus

    [CAT#: S01YF-0922-KX1592]
    Product Category:
    Membrane Protein Tools
    Subcategory:
    Membrane Protein Lentivirus

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    Certificate of Analysis Lookup
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    Product Information

    Product Overview
    The lentivirus product encodes Human ADRB3. To produce lentivirus particles, the transfer plasmid containing the Human ADRB3, the envelope plasmid, and the packaging plasmid are co-transfected into the packaging cell line. The lentivirus particles are purified and concentrated using sucrose gradient ultracentrifugation to ensure quality. Viral titer is then determined using RT-qPCR with primers to the elements present in the viral genome. The viral particles are shipped overnight and can be used immediately for in vitro or in vivo research. The lentiviruses are packaged using 3rd generation lentiviral plasmids, and replication-incompetent.
    Target Protein
    ADRB3
    Target Classificationp
    GPCR
    Target Family
    Adrenergic Family
    Target Protein Species
    Human
    Protein Tag
    Based on specific requirements (Flag, StrepII, His, etc.)
    Buffer
    HBSS
    Serotype
    HIV-1 or based on specific requirements
    Titer
    ≧1.00E+8 TU/mL
    Transfer Plasmid Backbone
    pCDH or specific requirements
    Size
    100 uL*10 vials, 1 mL
    Fluorescent Markers
    EGFP, mCherry etc.
    Selection Markers
    Puromycin, Hygromycin, Neomycin, Blasticidin etc.
    Target Research Area
    CNS Research; Metabolic Research; Cardiovascular Research
    Related Diseases
    Body Mass Index Quantitative Trait Locus; Low Compliance Bladder

    Quality Control

    Titer Determination Method
    RT-qPCR
    Sterility Testing
    Negative
    Mycoplasma Testing
    Negative
    Transfection Efficiency
    Positive (if EGFP is included in the transfer plasmid)
    Endotoxin Level
    Based on specific requirements

    Product Properties

    Application
    Stable cell line generation; Protein expression; Biochemical assays
    Safety Notes
    Since the HIV genes are expressed from packaging plasmids devoid of the packing signal, none of the HIV genes will be expressed in the transduced cells. The pseudotyped lentiviruses need to be used in a Biosafety Level 2 facility even if they are replication-incompetent.
    Shipping
    Dry ice
    Storage
    Store at -80°C and avoid repeated freeze-thaw cycles.

    Target

    Full Name
    Adrenoceptor beta 3
    Introduction
    The protein encoded by this gene belongs to the family of beta adrenergic receptors, which mediate catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor is located mainly in the adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Obesity and bodyweight-related disorders are correlated with certain polymorphisms in three subtypes of beta-adrenoceptor, among them, the ADRB3 gene.
    Alternative Names
    BETA3AR; adrenergic, beta-3-, receptor; beta-3 adrenoceptor; beta-3 adrenoreceptor; ADRB3; Adrenoceptor beta 3
    Gene ID
    UniProt ID

    Protocols

    Please visit our protocols page.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR CLINICAL PROCEDURES" For licensing inquiries, please contact
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