mProX™ Human ADORA1 Stable Cell Line

Product Information

Target Protein

ADORA1

Target Family

Adenosine Family

Target Protein Species

Human

Host Cell Type

A375;SK-MEL-28;CHO-K1;HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Cardiovascular Research

Related Diseases

Ischemia;Congestive Heart Failure.

Gene ID

Human: 134

UniProt ID

Human: P30542

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Adenosine receptor A1 (ADORA1) is a significant component in the realm of scientific research, particularly in the context of neurology and oncology. One of the notable findings is its link to early-onset Parkinson's disease, where a mutation in ADORA1 is associated with dysfunction in adenosine receptor heteromerization, potentially leading to a hyperglutamatergic state. Additionally, in the field of oncology, ADORA1 has been implicated in hepatocellular carcinoma (HCC). The recruitment of plasmacytoid dendritic cells, influenced by ADORA1 signaling, has been shown to induce immunosuppression in HCC. Targeting this recruitment could serve as a potential adjuvant strategy for immunotherapies in HCC. Furthermore, ADORA1's role in tumor immune evasion has been explored, where its inhibition can promote the effects of certain treatments in melanoma and NSCLC xenografts source. These findings underscore the importance of ADORA1 in various pathological conditions and its potential as a therapeutic target.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Charles (Verified Customer)

What is the significance of ADORA1 in stabilized cell line research? Jan 02 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

ADORA1 plays a crucial role in various cellular processes and has been the subject of numerous studies to understand its function and potential applications in research. Jan 02 2023

chat Brian (Verified Customer)

Are there any known challenges or misconceptions related to ADORA1 stabilized cell lines? Mar 10 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

While ADORA1 stabilized cell lines offer valuable insights, it's essential to ensure the accuracy of the stabilization process to maintain the integrity of the research findings. Mar 10 2022

Published Data

Fig.1 FACS of PD-L1+ membrane expression

A lack of Adora1 precipitated a pronounced augmentation of tumor PD-L1, as corroborated within melanoma cellular matrices in vitro. Demonstrations indicated notably elevated PD-L1 mRNA and protein quantities in specific groups compared to a control cohort.

Ref: Liu, Hong, et al. "ADORA1 inhibition promotes tumor immune evasion by regulating the ATF3-PD-L1 axis." Cancer cell 37.3 (2020): 324-339.

Pubmed: 32183950

DOI: 10.1016/j.ccell.2020.02.006

Research Highlights

Liu Y, et al. "Symptom-oriented network pharmacology revealed the mechanism of HuangQi-DanShen ." Journal of ethnopharmacology, 2024.
The authors conducted a study to elucidate the mechanism of HuangQi-DanShen (HD), an important drug pair in traditional Chinese medicine, for the treatment of cerebral ischemia (CI). They used UHPLC-Q-Exactive Orbitrap-MS technology to identify 190 ingredients in HD and then established a strategy to identify its chemical constitutes. Additionally, they employed a network pharmacology technique to elucidate the molecular mechanisms behind the symptom relief provided by HD for CI. Their findings suggest that HD regulates key factors such as ADORA2A, ADORA1, PTPN11, MMP9, EGFR, and CA2 to mitigate symptoms of CI. Further research using this approach may provide valuable insights into the therapeutic effects of HD on CI.
Pubmed: 37437791 DOI: 10.1016/j.jep.2023.116845

Zhang Y, et al. "A novel extrachromosomal circular DNA related genes signature for overall ." BMC medical genomics, 2023.
Ovarian cancer (OV), a highly lethal disease, is attributed to extrachromosomal circular DNA (eccDNA) and its role in carcinogenesis. The objective of this study is to investigate the molecular structure of eccDNA in the UACC-1598-4 cell line and the association of its genes with OV prognosis. The eccDNA was sequenced and annotated using Circle_seq, and genes were identified by intersecting annotated eccDNA genes with overexpressed genes in OV from TCGA. Cox regression, LASSO, and ROC analysis were used to create a 9-gene prognostic model. Results from TCGA and ICGC databases demonstrate the model's accuracy in distinguishing high-risk patients. Additionally, immune infiltration was found to vary significantly between high-risk and low-risk groups. This study concludes that eccDNA in UACC-1598-4 contains genes linked to OV prognosis, making the prognostic model based on these genes a potential tool for evaluating patients with OV.
Pubmed: 37337170 DOI: 10.1186/s12920-023-01576-x