Magic™ Human ADORA1 Lentivirus
[CAT#: S01YF-0922-KX1580]
- Product Category:
- Membrane Protein Tools
- Subcategory:
- Membrane Protein Lentivirus
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Information
Product Overview
The lentivirus product encodes Human ADORA1. To produce lentivirus particles, the transfer plasmid containing the Human ADORA1, the envelope plasmid, and the packaging plasmid are co-transfected into the packaging cell line. The lentivirus particles are purified and concentrated using sucrose gradient ultracentrifugation to ensure quality. Viral titer is then determined using RT-qPCR with primers to the elements present in the viral genome. The viral particles are shipped overnight and can be used immediately for in vitro or in vivo research. The lentiviruses are packaged using 3rd generation lentiviral plasmids, and replication-incompetent.
Target Protein
ADORA1
Target Classificationp
GPCR
Target Family
Adenosine Family
Target Protein Species
Human
Protein Tag
Based on specific requirements (Flag, StrepII, His, etc.)
Buffer
HBSS
Serotype
HIV-1 or based on specific requirements
Titer
≧1.00E+8 TU/mL
Transfer Plasmid Backbone
pCDH or specific requirements
Size
100 uL*10 vials, 1 mL
Fluorescent Markers
EGFP, mCherry etc.
Selection Markers
Puromycin, Hygromycin, Neomycin, Blasticidin etc.
Target Research Area
CNS Research; Cardiovascular Research; Reproductive Research
Related Diseases
Pertussis; Ischemia
Quality Control
Titer Determination Method
RT-qPCR
Sterility Testing
Negative
Mycoplasma Testing
Negative
Transfection Efficiency
Positive (if EGFP is included in the transfer plasmid)
Endotoxin Level
Based on specific requirements
Product Properties
Application
Stable cell line generation; Protein expression; Biochemical assays
Safety Notes
Since the HIV genes are expressed from packaging plasmids devoid of the packing signal, none of the HIV genes will be expressed in the transduced cells. The pseudotyped lentiviruses need to be used in a Biosafety Level 2 facility even if they are replication-incompetent.
Shipping
Dry ice
Storage
Store at -80°C and avoid repeated freeze-thaw cycles.
Target
Full Name
Adenosine A1 receptor
Introduction
The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene.
Alternative Names
RDC7; adenosine A1 receptor variant 1; adenosine A1 receptor variant 2; AA1R; ADORA1; Adenosine A1 receptor
Gene ID
UniProt ID
Protocols
Please visit our protocols page.
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