Human HLA-A Membrane Protein Lentivirus

Titer Determination Method

RT-qPCR

Sterility Testing

Negative

Mycoplasma Testing

Negative

Transfection Efficiency

Positive (if EGFP is included in the transfer plasmid)

Endotoxin Level

Based on specific requirements

Application

Stable cell line generation; Protein expression; Biochemical assays

Safety Notes

Since the HIV genes are expressed from packaging plasmids devoid of the packing signal, none of the HIV genes will be expressed in the transduced cells. The pseudotyped lentiviruses need to be used in a Biosafety Level 2 facility even if they are replication-incompetent.

Shipping

Dry ice

Storage

Store at -80°C and avoid repeated freeze-thaw cycles.

Full Name

Major histocompatibility complex, class I, A

Introduction

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen so that they can be recognized by cytotoxic T cells. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. More than 6000 HLA-A alleles have been described. The HLA system plays an important role in the occurrence and outcome of infectious diseases, including those caused by the malaria parasite, the human immunodeficiency virus (HIV), and the severe acute respiratory syndrome coronavirus (SARS-CoV). The structural spike and the nucleocapsid proteins of the novel coronavirus SARS-CoV-2, which causes coronavirus disease 2019 (COVID-19), are reported to contain multiple Class I epitopes with predicted HLA restrictions. Individual HLA genetic variation may help explain different immune responses to a virus across a population.

Alternative Names

HLA-A; HLAA; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; MHC class I antigen HLA-A heavy chain; leukocyte antigen class I-A; Major histocompatibility complex, class I, A

Gene ID

UniProt ID