mProX™ Human VIPR2 Stable Cell Line

Product Information

Target Protein

VIPR2

Target Family

VIP/PACAP Family

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

CNS Research; Cardiovascular Research

Related Diseases

Schizophrenia; Holoprosencephaly

Gene ID

7434

UniProt ID

P41587

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

VIPR2 is a seven transmembrane heterotrimeric GPCR. It has received a lot of attention in recent years due to the multiple therapeutic potential it presents. Numerous physiological processes, including glucose homeostasis, neuroprotection, memory, gastrointestinal function, immune system regulation, and circadian rhythm, have been linked to VIPR2 receptors, according to studies. Two homologous neuropeptides, PACAP and VIP, can bind to the VIPR2 protein-coupled receptor with a high affinity. On active CD4+ T cells and monocytes, VIPR2 is best expressed. Researchers have previously hinted that VIPR2 plays a significant role in a number of diseases, including schizophrenia, breast cancer, and a number of psychiatric disorders. These clinical trials show it might function as a biomarker in clinical settings. The customized VIPR2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Tracy

mProX™ Human VIPR2 Stable Cell Line is very good! Jan 27 2023

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chat Viola

I purchased the cell line. Hope it will work well. Jun 22 2023

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FAQ

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Published Data

Fig.1 Gpr176 basal activity inhibits Vip-Vipr2-cAMP signalling.

Gpr176 and Vipr2 immunoblots using cell membrane fractions from TREx293-Flp-In tet-on/Vipr2 cells. For 24 hours, cells were given either 1 g ml-1 Dox or a control substance. Confocal pictures of Gpr176 and Vipr2 immunofluorescent labeling in Flp-In TREx293-Gpr176(tet-on)/Vipr2 cells treated or untreated with Dox.

Ref: Doi, Masao, et al. "Gpr176 is a Gz-linked orphan G-protein-coupled receptor that sets the pace of circadian behaviour." Nature communications 7.1 (2016): 10583.

Pubmed: 26882873

DOI: 10.1038/ncomms10583

Research Highlights

According to in vitro research, PACAP and VIP use separate signaling pathways to influence the development of neurons in various ways. As a result, changes in the ratio of VPAC2, PAC1, and PAC1 receptors and their ligands may have significant effects on how the brain develops and changes over time.
Ago, Yukio, et al. "Probing the VIPR2 microduplication linkage to schizophrenia in animal and cellular models." Frontiers in Neuroscience 15 (2021): 717490.
Pubmed: 34366784 DOI: 10.3389/fnins.2021.717490

These preliminary findings show that peripheral DNA methylation indicators in ADHD may be interesting and propose focused ideas for further investigation in bigger samples, even though it is uncertain to what extent CpG methylation detected in peripheral tissue reflects transcriptome changes in the brain.
Wilmot, Beth, et al. "Methylomic analysis of salivary DNA in childhood ADHD identifies altered DNA methylation in VIPR 2." Journal of Child Psychology and Psychiatry 57.2 (2016): 152-160.
Pubmed: 26304033 DOI: 10.1111/jcpp.12457