mProX™ Human TRPV4 Stable Cell Line

Product Information

Target Protein

TRPV4

Target Family

TRPV

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research; Cardiovascular Research

Related Diseases

Spondylometaphyseal Dysplasia; Scapuloperoneal Spinal Muscular Atrophy

Gene ID

59341

UniProt ID

Q9HBA0

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The TRPV4 gene in humans encodes the ion channel protein known as transient receptor potential cation channel subfamily V member 4. The encoded protein is a nonselective, Ca2+-permeable cation channel that has been linked to a number of physiological dysfunctions, disorders, and diseases. It has a variety of roles, including the brain's control over systemic osmotic pressure, vascular, liver, intestinal, renal, and bladder functions, skin barrier and UVB radiation response, skeletal growth and structural integrity, joint function, airway and lung function, retinal and inner ear function, and pain. Chemical, mechanical, and osmotic stimuli all work to open the channel. It reacts to temperature variations as well. The customized TRPV4 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Paul

This cell line is very suitable for studying the TRPV4 signaling pathway. Mar 27 2021

chat Verified Customer

chat Jessica

The TRPV4 cell line is suitable for our drug screening. Feb 21 2022

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Comparison of TRPV4 current activation by GSK1016790A and 4α-PDD.

Concentration-dependent increases in current density in TRPV4-expressing HEK293 cells were evaluated at +60 mV using GSK1016790A and 4α-PDD.

Ref: Thorneloe, Kevin S., et al. "N-((1S)-1-{[4-((2S)-2-{[(2, 4-dichlorophenyl) sulfonyl] amino}-3-hydroxypropanoyl)-1-piperazinyl] carbonyl}-3-methylbutyl)-1-benzothiophene-2-carboxamide (GSK1016790A), a novel and potent transient receptor potential vanilloid 4 channel agonist induces urinary bladder contraction and hyperactivity: Part I." Journal of Pharmacology and Experimental Therapeutics 326.2 (2008): 432-442.

Pubmed: 18499743

DOI: 10.1124/jpet.108.139295

Research Highlights

Numerous critical physiological functions of TRPV4 are fulfilled, and its aberrant functioning or absence can be linked to a variety of disease conditions.
White, John PM, et al. "TRPV4: molecular conductor of a diverse orchestra." Physiological reviews 96.3 (2016): 911-973.
Pubmed: 27252279 DOI: 10.1152/physrev.00016.2015

Biomechanical variables have been linked to the development of osteoarthritis and are essential in controlling the physiology and pathology of various joint tissues.
McNulty, Amy L., et al. "TRPV4 as a therapeutic target for joint diseases." Naunyn-Schmiedeberg's archives of pharmacology 388 (2015): 437-450.
Pubmed: 25519495 DOI: 10.1007/s00210-014-1078-x