mProX™ Human TRPV3 Stable Cell Line

Product Information

Target Protein

TRPV3

Target Family

TRPV

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research; Cardiovascular Research

Related Diseases

Palmoplantar Keratoderma; Nonepidermolytic; Olmsted Syndrome

Gene ID

162514

UniProt ID

Q8NET8

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The protein of the Transient Receptor Potential Cation Channel, Subfamily V, Member 3 is encoded by the human gene TRPV3. Widespread tissue expression of the TRPV3 channel is particularly high in the skin and brain. It serves as a molecular temperature sensor in safe, heated environments. Mice that lack these proteins can feel cold and uncomfortable heat, but not high temperatures. TRPV3 channels appear to be involved in hair growth in addition to thermosensation because mutations in the TRPV3 gene cause hair loss in mice. Though its exact function in the brain is unknown, TRPV3 channels seem to be involved in mood regulation. TRPV3 acted as a partial mediator of the natural substance incensole acetate's protective properties. The customized TRPV3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Gary

Construct works beautifully, definitely will get more if needed. Apr 22 2020

chat Verified Customer

chat David

I customized a TRPV3 cell line and it's very good. Sep 06 2023

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Functional characterization of the TRPV3 wild-type channels.

Sample whole-cell current traces from TRPV3 wild-type and T96A channels recorded at +60 mV. The channels were stimulated by applying 30 µM 2-APB repeatedly for 15 s, followed by total washout (left) and the matching time course of sensitization from the recording (right).

Ref: Zubcevic, Lejla, et al. "Conformational ensemble of the human TRPV3 ion channel." Nature Communications 9.1 (2018): 4773.

Pubmed: 30429472

DOI: 10.1038/s41467-018-07117-w

Research Highlights

In both skin physiology and pathology, the transient receptor potential vanilloid subfamily member 3 (TRPV3) channel is essential. Atopic dermatitis, rosacea, and Olmsted syndrome are among the skin conditions linked to mutations in TRPV3.
Singh, Appu K., Luke L. McGoldrick, and Alexander I. Sobolevsky. "Structure and gating mechanism of the transient receptor potential channel TRPV3." Nature structural & molecular biology 25.9 (2018): 805-813.
Pubmed: 30127359 DOI: 10.1038/s41594-018-0108-7

Important roles for the TRPV3 channel are played in skin physiology. TRPV3 dysfunction results in cutaneous conditions, such as Olmsted syndrome. Nevertheless, TRPV3's validity as a therapeutic target is hampered by the absence of strong and specific inhibitors.
Fan, Junping, et al. "Structural basis of TRPV3 inhibition by an antagonist." Nature Chemical Biology 19.1 (2023): 81-90.
Pubmed: 36302896 DOI: 10.1038/s41589-022-01166-5