mProX™ Human TRPM2 Stable Cell Line

Product Information

Target Protein

TRPM2

Target Family

TRPM

Target Protein Species

Human

Host Cell Type

HEK 293; MDA-MB-231; MCF-7; CHO-K1

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research; Autoimmune Research

Related Diseases

Innate Immune System

Gene ID

7226

UniProt ID

O94759

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

A non-selective cation channel that is permeable to calcium (Ca2+) is called transient receptor potential melastatin 2 (TRPM2). Over the past two decades, mechanisms controlling TRPM2 have been identified, and elements that enhance or decrease the channel's functionality have become more clear. Nevertheless, little is known about the mechanisms by which these variables dynamically control TRPM2 function in both health and illness. Among transient receptor potential (TRP) channels, TRPM2 mRNA is the most prevalent in the central nervous system (CNS)2. Numerous significant contributions of TRPM2 channels to CNS physiology and pathology have been identified by an expanding body of research. The customized TRPM2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Amanda

I purchased the Human TRPM2 Stable Cell Line for high-throughput drug screening. Jul 08 2021

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chat Cynthia

This cell underwent a lot of QC analysis. Jun 07 2020

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FAQ

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Published Data

Fig.1 TRPM2 levels in breast cell lines and knockdown of TRPM2 levels by RNAi silencing.

Immunoblot analysis of the levels of the protein TRPM2 in two malignant and two non-cancerous breast cell lines (MCF-7, MDA-MB-231) and HMEC, MCF-10A. The positive control was the quantity of TRPM2 protein detected by immunoblot analysis in human embryonic kidney cells (HEK 293).

Ref: Hopkins, Mandi M., et al. "Inhibition of the transient receptor potential melastatin-2 channel causes increased DNA damage and decreased proliferation in breast adenocarcinoma cells." International journal of oncology 46.5 (2015): 2267-2276.

Pubmed: 25760245

DOI: 10.3892/ijo.2015.2919

Research Highlights

TRPM2, also known as transient receptor potential melastatin 2, is a cation channel linked to a number of illnesses. Adenosine diphosphate (ADP)-ribose (ADPR) is bound by its C-terminal NUDT9 homology (NUDT9H) domain, and TRPM2 activation requires both ADPR and Ca2+.
Wang, Longfei, et al. "Structures and gating mechanism of human TRPM2." Science 362.6421 (2018): eaav4809.
Pubmed: 30467180 DOI: 10.1126/science.aav4809

The activity of TRP-melastatin 2 (TRPM2), which is dependent on intracellular calcium (Ca2+), is specifically engaged in the response to oxidative stress and inflammation. Through the control of various signaling pathways, TRPM2 is involved in a number of physiological and pathological processes in the brain.
Sita, Giulia, et al. "TRPM2 in the brain: role in health and disease." Cells 7.7 (2018): 82.
Pubmed: 30037128 DOI: 10.3390/cells7070082