mProX™ Human TRPC6 Stable Cell Line

Product Information

Target Protein

TRPC6

Target Family

TRPC

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research; Digestive and Renal Research

Related Diseases

Focal Segmental Glomerulosclerosis; Nephrotic Syndrome

Gene ID

7225

UniProt ID

Q9Y210

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

To encourage vasoconstriction, mechanosensation, such as cell enlargement and shear stress, can activate TRPC6 channels. GPCRs can also activate these channels. For instance, 1 nM ANG II administration activates TRPC6 channels by a process that is not dependent on PKC and is directly linked to diacylglycerol. In a fascinating work published more recently, it was suggested that Ca2 + entry via TRPC6 is essential to a force-sensing complex that supports Ca2 + release via IP3Rs on the SR to increase myogenic tone and TRPM4 channel activity. Even yet, more research is required to determine this channel's contributions to local and global Ca2 + signals, function in various vascular beds, and activation and mechanosensation mechanisms in VSM, despite the abundance of information available on it. The customized TRPC6 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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This cell line is perfect for my experiments. Jun 18 2021

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Professional products and professional services. Dec 25 2022

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FAQ

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Published Data

Fig.1 TRPC6 channel response in TRPC6-expressing HEK293T cells.

The levels of TRPC6 protein expression in untransfected (UT) HEK293T cells and in HEK293T cells transfected with a vector (Mock), rat TRPC6 expression plasmid (rTRPC6), and rTRPC6 plus RNAi constructs for rat TRPC6 (rTRPC6rTRPC6-shRNA) are displayed in a western blot.

Ref: Graham, Sarabeth, et al. "Canonical transient receptor potential 6 (TRPC6), a redox-regulated cation channel." Journal of Biological Chemistry 285.30 (2010): 23466-23476.

Pubmed: 20501650

DOI: 10.1074/jbc.M109.093500

Research Highlights

In an effort to shed light on the mechanisms behind the pathophysiology of TRPC6-mediated glomeruli damage and its potential significance as a therapeutic target for the treatment of chronic kidney illnesses, this mini-review concentrates on the TRPC6 channel in podocytes and collates recent evidence.
Ilatovskaya, Daria V., and Alexander Staruschenko. "TRPC6 channel as an emerging determinant of the podocyte injury susceptibility in kidney diseases." American Journal of Physiology-Renal Physiology 309.5 (2015): F393-F397.
Pubmed: 26084930 DOI: 10.1152/ajprenal.00186.2015

Nonselective cation channels formed by transient receptor potential canonical (TRPC) proteins are involved in a range of physiological processes in different types of cells. The molecular knowledge of TRPC6 activity and modulation is still unclear, despite mounting data indicating the therapeutic potential of TRPC6 inhibition in treating pathological cardiac and renal diseases.
Bai, Yonghong, et al. "Structural basis for pharmacological modulation of the TRPC6 channel." Elife 9 (2020): e53311.
Pubmed: 32149605 DOI: 10.7554/eLife.53311