mProX™ Human TRPC4 Stable Cell Line

Product Information

Target Protein

TRPC4

Target Family

TRPC

Target Protein Species

Human

Host Cell Type

U266; MM.1s; HS-5; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

CNS Research; Pain and Addiction Research

Related Diseases

Oculoectodermal Syndrome; Mutilating Palmoplantar Keratoderma

Gene ID

7223

UniProt ID

Q9UBN4

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Whether TRPC4 serves as a SOC or ROC is a matter of debate. It has been proposed that AA activates TRPC4 to generate a ROC. Specifically, by employing siRNA and antisense tactics to decrease endogenous TRPC4 expression, it was demonstrated that TRPC4 is necessary for both receptor-operated and OAG-induced Ca2+ entry as well as AA-induced Ca2+ oscillations, but not for SOC function. Since AA has been found to activate a number of MS channels in the absence of applied stretch, where it appears to work by directly modifying the mechanical properties of the bilayer surrounding the channel, this activation of AA may have consequences for the mechanosensitivity of TRPC4. The customized TRPC4 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The web-page is easy to navigate in order to choose or customize the product. Feb 12 2020

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The Human TRPC4 Stable Cell Line is perfect for my research. Jan 05 2021

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 TRPC4 channel contributes to MTI-101 induced Ca2+/Na+ influx and cell death.

Relative expression levels of TRPC1, TRPC4, and TRPC5 mRNA in U266, MM.1s, and HS-5 cell lines. In each cell line, the amount of expression of the corresponding mRNA is displayed in relation to GAPDH.

Ref: Elzamzamy, Osama M., et al. "Transient receptor potential C 1/4/5 is a determinant of MTI-101 induced calcium influx and cell death in multiple myeloma." Cells 10.6 (2021): 1490.

Pubmed: 34199280

DOI: 10.3390/cells10061490

Research Highlights

Novel pharmacological targets and techniques are highly sought for, as current medicines for common cancer types, such as renal cell cancer, are frequently unspecific and unsuccessful.
Akbulut, Yasemin, et al. "(−)-Englerin A is a potent and selective activator of TRPC4 and TRPC5 calcium channels." Angewandte Chemie International Edition 54.12 (2015): 3787-3791.
Pubmed: 25707820 DOI: 10.1002/anie.201411511

Gaining more understanding of the molecular underpinnings of TRPC channel activation can be facilitated by identifying the regulatory role of the NHERF proteins in the TRPC4/5-NHERF protein complex. Subsequent investigations must elucidate the physiological and pathological roles of this multi-protein channel complex.
Mederos y Schnitzler, Michael, Thomas Gudermann, and Ursula Storch. "Emerging roles of diacylglycerol-sensitive TRPC4/5 channels." Cells 7.11 (2018): 218.
Pubmed: 30463370 DOI: 10.3390/cells7110218