mProX™ Human SLC6A4 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-1122-KX208	Magic™ Human SLC6A4 in Vitro Radioligand Binding Assay	Human	HEK293	Radioligand Binding Assay

Product Information

Target Protein

SLC6A4

Target Family

SLC Transporter

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Transporter Cell Lines

Target Research Area

CNS Research

Related Diseases

Obsessive-Compulsive Disorder; Anxiety

Gene ID

6532

UniProt ID

P31645

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The SLC6A4 gene in humans encodes the serotonin transporter, sometimes referred to as the sodium-dependent serotonin transporter and solute carrier family 6 member 4. Serotonin reuptake is the process by which the monoamine transporter protein SERT returns the neurotransmitter serotonin from the synaptic cleft to the presynaptic cell. Serotonin is transported by the SERT protein, which then recycles it in a way that is dependent on sodium to stop its effects. In order to decrease serotonin reuptake, many antidepressant drugs from the SSRI and tricyclic antidepressant families bind to SERT. The customized SLC6A4 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Timothy

As a researcher in vascular biology, I can confidently say that the SLC6A4 cell line is a fantastic tool. Oct 26 2021

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chat Helen

This SLC6A4 cell line is the best choice for laboratories wishing to minimise troubleshooting time and maximise data and experimental volumes. Sep 13 2022

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 CTCF modulates expression of the endogenous SLC6A4 in JAr cells.

Q-PCR analysis of the mRNA expression of CTCF and SLC6A4. On 10 5 JAr cells, CTCF siRNA experiments were conducted together with transfection using the CTCF expressing vector. 48 hours after transfection, RNA was extracted, cDNA was made, and Q-PCR was carried out.

Ref: Roberts, Julian, et al. "Differential regulation of the serotonin transporter gene by lithium is mediated by transcription factors, CCCTC binding protein and Y-box binding protein 1, through the polymorphic intron 2 variable number tandem repeat." Journal of Neuroscience 27.11 (2007): 2793-2801.

Pubmed: 17360901

DOI: 10.1523/JNEUROSCI.0892-06.2007

Research Highlights

It has been established that SLC6A4 is a significant indicator of early adversity exposures, and epigenetic processes at this gene seem to be essential for programming.
Provenzi, Livio, et al. "SLC6A4 methylation as an epigenetic marker of life adversity exposures in humans: a systematic review of literature." Neuroscience & Biobehavioral Reviews 71 (2016): 7-20.
Pubmed: 27565518 DOI: 10.1016/j.neubiorev.2016.08.021

Stress in both early and recent life affects DNA methylation according to 5-HTTLPR genotype. Certain variations in stress response are also mirrored in gene expression and the cortisol response, which may contribute to an individual's susceptibility or resilience to psychopathology when they encounter stressful life events.
Duman, Elif A., and Turhan Canli. "Influence of life stress, 5-HTTLPR genotype, and SLC6A4 methylation on gene expression and stress response in healthy Caucasian males." Biology of mood & anxiety disorders 5 (2015): 1-14.
Pubmed: 25995833 DOI: 10.1186/s13587-015-0017-x