mProX™ Human SLC6A2 Stable Cell Line

Product Information

Target Protein

SLC6A2

Target Family

SLC Transporter

Target Protein Species

Human

Host Cell Type

SK-N-BE(2)C; SK-N-BE(2)N; CHO-K1; HEK293

Target Classification

Transporter Cell Lines

Target Research Area

CNS Research

Related Diseases

Disease; Nuclear receptors meta-pathway

Gene ID

6530

UniProt ID

P23975

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The SLC6A2 gene in humans encodes a protein called the norepinephrine transporter (NET), also referred to as the noradrenaline transporter (NAT). The sodium-chloride (Na+/Cl−)-dependent reuptake of extracellular norepinephrine (NE), also referred to as noradrenaline, is carried out by the monoamine transporter NET. Furthermore, extracellular dopamine (DA) can be reabsorbed by NET. The regulation of concentrations in the synaptic cleft is contingent upon the reuptake of these two neurotransmitters. Many antidepressants and recreational drugs target NETs and the other monoamine transporters. Additionally, ADHD is linked to an excess of NET. Evidence points to the NET gene (SLC6A2) single-nucleotide polymorphisms as a potential underlying cause of some of these illnesses. The customized SLC6A2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The SLC6A2 cell line has exceeded my expectations in terms of performance and has become an invaluable asset in our lab. Jul 14 2020

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The SLC6A2 cell line has become a crucial tool in our lab, helping us gain deeper insights into angiogenesis and related processes. Jun 17 2022

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FAQ

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Published Data

Fig.1 The expression of NET protein in neuroblastoma cell lines.

Western blotting and 123I-MIBG uptake assays were used to evaluate the expression of NET in five human neuroblastoma cell lines. The endogenous expression of NET was shown to be high in SK-N-BE(2)C cells, moderate in SK-N-BE(2)N and SK-N-BE(1)N cells, and low in LAN1 and SK-N-SH cells, according to both tests.

Ref: Zhang, Hanwen, et al. "Imaging the norepinephrine transporter in neuroblastoma: a comparison of [18F]-MFBG and 123I-MIBG." Clinical Cancer Research 20.8 (2014): 2182-2191.

Pubmed: 24573553

DOI: 10.1158/1078-0432.CCR-13-1153

Research Highlights

This investigation looked at potential associations between remission following venlafaxine medication for major depressive disorder (MDD) and polymorphisms in the norepinephrine transporter gene (SLC6A2).
Yeh, Yi-Wei, et al. "SLC6A2 variants may predict remission from major depression after venlafaxine treatment in Han Chinese population." Journal of psychiatric research 61 (2015): 33-39.
Pubmed: 25512257 DOI: 10.1016/j.jpsychires.2014.11.017

This work sheds light on the function of microRNAs in the etiopathogenesis of anxiety disorders, namely those involving the cardiovascular system and its comorbidities.
Hommers, Leif G., et al. "A functional genetic variation of SLC6A2 repressor hsa-miR-579-3p upregulates sympathetic noradrenergic processes of fear and anxiety." Translational psychiatry 8.1 (2018): 226.
Pubmed: 30341278 DOI: 10.1038/s41398-018-0278-4