mProX™ Human SLC47A2 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types	Inquiry	Datasheet
S01YF-0922-KX1568	Magic™ Human SLC47A2 in Vitro Drug screening assay	Human	Caco-2, MDCK, HEK293, CHO, membrane vesicles, and various transfected cells lines	Activity Assay (High throughput screening assays; Large scale single dose, duplicate profiling; IC50 profiling; Ki determination assay; Substrate determination assay )

Product Information

Target Protein

SLC47A2

Target Family

SLC Transporter

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Transporter Cell Lines

Target Research Area

Metabolic Research

Related Diseases

Thiamine Metabolism Dysfunction Syndrome

Gene ID

146802

UniProt ID

Q86VL8

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human gene SLC47A2 encodes the protein known as multidrug and toxin extrusion protein 2. This gene produces a protein that is a member of a family of transporters that are involved in the excretion of toxic electrolytes through the bile and urine, both endogenous and exogenous. The bacterial MATE (multi antimicrobial extrusion protein or multidrug and toxic chemical extrusion) protein family, which is in charge of drug resistance, and this transporter family are homologous. On chromosome 17, this gene is one of two MATE transporter family members that are close to one another. For this gene, alternatively spliced transcript variants encoding several isoforms have been found. The customized SLC47A2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Donna

The SLC47A2 cell line was easy to use, saving me valuable time in the lab. May 26 2020

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chat Christopher

I am truly impressed with the performance of this SLC47A2 cell line and the professionalism of Creative Biolabs. I highly recommend it to anyone. Oct 17 2021

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FAQ

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Published Data

Fig.1 SANT1 overexpression enhanced SLC47A2 expression in RCC cell lines.

After transfecting the SANT1 plasmid into the 786-O, ACHN, 769-P, and Caki-1 cell lines, 48 hours were allowed for the extraction of total RNA and protein. Remarkably, regardless of cellular heterogeneities, SANT1 markedly increased the expression of SLC47A2 in all four cell lines.

Ref: Gao, Zhangzhao, et al. "A novel human lncRNA SANT1 cis-regulates the expression of SLC47A2 by altering SFPQ/E2F1/HDAC1 binding to the promoter region in renal cell carcinoma." RNA biology 16.7 (2019): 940-949.

Pubmed: 30951404

DOI: 10.1080/15476286.2019.1602436

Research Highlights

The SNPs in the SLC47A1 and SLC47A2 genes, which code for the multidrug and toxin extrusion protein (MATE) transporters, were not linked to the clinical response to metformin. Moreover, neither the dosage required nor the side effects of metformin were impacted by the investigated SNPs.
Raj, Gerard Marshall, et al. "Lack of effect of the SLC47A1 and SLC47A2 gene polymorphisms on the glycemic response to metformin in type 2 diabetes mellitus patients." Drug Metabolism and Personalized Therapy 33.4 (2018): 175-185.
Pubmed: 30433870 DOI: 10.1515/dmpt-2018-0030

The pharmacokinetics of metformin were found to be connected to both exonic single nucleotide polymorphisms.
Moeez, Sadaf, et al. "Effects of SLC22A2 (rs201919874) and SLC47A2 (rs138244461) genetic variants on Metformin Pharmacokinetics in Pakistani T2DM patients." JPMA. The Journal of the Pakistan Medical Association 69.2 (2019): 155-163.
Pubmed: 30804576