mProX™ Human SLC30A8 Stable Cell Line

Product Information

Target Protein

SLC30A8

Target Family

SLC Transporter

Target Protein Species

Human

Host Cell Type

MEL1; CHO-K1; HEK293

Target Classification

Transporter Cell Lines

Target Research Area

Diabetes Research

Related Diseases

Type 2 Diabetes Mellitus; Type 1 Diabetes Mellitus

Gene ID

169026

UniProt ID

Q8IWU4

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The zinc transporter ZnT8 is encoded by SLC30A8. It is expressed in β cells and is in charge of transferring zinc into insulin granules, which is required for the packing and release of insulin. This gene has been linked to β-cell biology, but until GWAS linked the missense variant R325W to T2D, it was unknown that changes in this pathway may cause T2D. Further studies on animals revealed that when glucose or a high-fat diet is presented to mice lacking this gene, there are mild anomalies in the way insulin is secreted. Even in β-cell-specific knockout mice, these abnormalities are evident, and they can be attributed to function loss brought on by the risk R325 gene. However, an examination of the protein's crystal structure suggests that the suggested change of an amino acid may not have a significant impact on function. The customized SLC30A8 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Christopher

The SLC30A8 cell line worked flawlessly, and the detailed protocols provided by the company made the whole process much easier. Mar 13 2022

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chat Carol

The mouse SLC30A8 overexpression cell line was delivered promptly, and it performed exceptionally well in my assays. May 04 2020

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FAQ

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Published Data

Fig.1 SLC30A8 KO and R138X mutant stem cell-derived beta cells have comparable glucose regulation compared to WT.

Proinsulin secretion to insulin secretion ratio in mice transplanted with beta-like cells produced from R138X stem cells, WT, and KO cells. In SLC30A8 mutants, proinsulin processing was similar to WT, as evidenced by each line's comparable proinsulin to insulin production ratio.

Ref: Sui, Lina, et al. "ZnT8 Loss of Function Mutation Increases Resistance of Human Embryonic Stem Cell-Derived Beta Cells to Apoptosis in Low Zinc Condition." Cells 12.6 (2023): 903.

Pubmed: 36980244

DOI: 10.3390/cells12060903

Research Highlights

A zinc transporter that is predominantly expressed in the pancreatic islets of Langerhans is encoded by the SLC30A8 gene. It moves zinc into insulin-containing secretory granules in β-cells. Humans with loss-of-function (LOF) mutations in SLC30A8 are protected from type 2 diabetes.
Kleiner, Sandra, et al. "Mice harboring the human SLC30A8 R138X loss-of-function mutation have increased insulin secretory capacity." Proceedings of the National Academy of Sciences 115.32 (2018): E7642-E7649.
Pubmed: 30038024 DOI: 10.1073/pnas.1721418115

The secretory granule-resident and mostly endocrine pancreas-restricted zinc transporter ZnT8 is encoded by SLC30A8. Diabetologists became interested in this gene product in 2007 after variants in SLC30A8 were found to increase disease risk in the first genome-wide association study for type 2 diabetes.
Rutter, Guy A., and Fabrice Chimienti. "SLC30A8 mutations in type 2 diabetes." Diabetologia 58 (2015): 31-36.
Pubmed: 25287711 DOI: 10.1007/s00125-014-3405-7