mProX™ Human SCN3A Stable Cell Line

Product Information

Target Protein

SCN3A

Target Family

Voltage Gated Sodium Channel

Target Protein Species

Human

Host Cell Type

Neuro-2a; CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Related Diseases

Developmental And Epileptic Encephalopathy; Epilepsy

Gene ID

6328

UniProt ID

Q9NY46

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Nav1.3 channels, which are widely distributed in the brain, are increased and discovered in dorsal root ganglion (DRG) sensory neurons after axotomy and other types of peripheral nerve injury. The significance of Nav1.3 channels in neuropathic pain is still up for debate, and their role in inflammatory pain has not yet been determined. The expression of Nav1.3 sodium channels in tiny neurons of the lumbar DRG was elevated by acute inflammation brought on by carrageenan injection into the hind paw, while Nav1.7 and Nav1.8 sodium channels were not affected in the same way. Nav1.3 mRNA is highly expressed in DRG in embryonic (E17) but significantly less so in adult rats. The customized SCN3A stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Kevin (Verified Customer)

How is SCN3A in stable cell lines? Jan 31 2022

chat Sherry Smith (Creative Biolabs Scientific Support)

SCN3A plays a role in various cellular processes. Ensuring its stability in cell lines is essential for accurate research outcomes. Jan 31 2022

chat Charles (Verified Customer)

What are the challenges associated with using SCN3A stable cell lines? Jun 18 2021

chat Sherry Smith (Creative Biolabs Scientific Support)

Like other stabilized cell lines, ensuring the accurate stabilization of SCN3A is crucial. Any inconsistencies can impact the reliability of research findings. Jun 18 2021

Published Data

Fig.1 VPA induces downregulation of Scn3a expression in Neuro-2a cells.

NaV1.3 levels in Neuro-2a cells were downregulated by VPA, as shown by Western blots. The NaV1.3 concentrations were scaled to GAPDH. One of three distinct repeats is seen in the left image. The untreated control cells' relative protein levels (NaV1.3/GAPDH) were normalized to "1."

Ref: Tan, Na-Na, et al. "Epigenetic downregulation of Scn3a expression by valproate: a possible role in its anticonvulsant activity." Molecular neurobiology 54 (2017): 2831-2842.

Pubmed: 27013471

DOI: 10.1007/s12035-016-9871-9

Research Highlights

Lamar, Tyra, et al. "SCN3A deficiency associated with increased seizure susceptibility." Neurobiology of disease 102 (2017): 38-48.
Pubmed: 28235671 DOI: 10.1016/j.nbd.2017.02.006

Pathogenic variations in SCN3A, which codes for the voltage-gated sodium channel subunit Nav1.3, result in malformations of cortical development and severe epilepsy that manifests in childhood.
Zaman, Tariq, et al. "SCN3A-related neurodevelopmental disorder: A spectrum of epilepsy and brain malformation." Annals of neurology 88.2 (2020): 348-362.
Pubmed: 32515017 DOI: 10.1002/ana.25809