mProX™ Human S1PR2 Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
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Patrick Liam (Creative Biolabs Scientific Support)
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Published Data
Fig.1 Silencing S1PR2 hampers cPLA2 phosphorylation, a process potentiated by H2O2, through the reduction of p38 and Erk1/2 activation in bEnd3 cells.
Immunoblotting was performed on lysates from lentivirus-infected bEnd3 cells subjected to a 30-minute exposure of 2 mM H2O2, using specified antibodies for analysis.
Ref: Cao, Changchun, et al. "S1PR2 antagonist alleviates oxidative stress-enhanced brain endothelial permeability by attenuating p38 and Erk1/2-dependent cPLA2 phosphorylation." Cellular Signalling 53 (2019): 151-161.
Pubmed: 30290210
DOI: 10.1016/j.cellsig.2018.09.019
Research Highlights
Skoug C, et al. "Density of Sphingosine-1-Phosphate Receptors Is Altered in Cortical ." Neurochemical research, 2023.
The present study aimed to investigate the potential alterations in sphingosine-1-phosphate (S1P) signaling in nerve terminals of insulin-resistant and diet-induced obese animal models of type 2 diabetes (T2D). S1P is a phosphosphingolipid with diverse biological functions and has been reported to play a role in neurodegenerative disorders. Our previous research has shown that neurons possess S1P receptors (S1PRs) with distinct localization and neuromodulatory effects. The current study used synaptosomes from the cortex of Goto-Kakizaki (GK) rats and Wistar controls, as well as mice fed a high-fat diet (HFD) and low-fat controls, to determine the density of S1PRs. Results showed lower levels of S1PR1, S1PR2, and S1PR4 in nerve-terminal-enriched membranes of GK rats, despite similar levels of S1P in the cortex compared to controls. On the other hand, HFD-fed mice exhibited increased levels of S1P in both plasma and cortex, along with decreased density of S1PR1 and S1PR4. These findings indicate altered S1P signaling in synapses of insulin resistance and obesity models, suggesting a potential role of S1P in the synaptic dysfunction associated with T2D.
Pubmed:
37794263
DOI:
10.1007/s11064-023-04033-4
Kim H, et al. "Clinical Significance of Sphingosine 1-phosphate Receptor 2 and Takeda G ." Journal of gastrointestinal and liver diseases : JGLD, 2023.
In biliary epithelial cells, two bile acid receptors, S1PR2 and TGR5, have been found to promote cell proliferation and invasive behavior in cancerous cells. The purpose of this study was to determine the clinical relevance of S1PR2 and TGR5 expression in patients with extrahepatic cholangiocarcinoma (CCA). A total of 115 patients who underwent surgical resection at Korea University Guro Hospital from 2002 to 2018 were included. The expression levels of S1PR2 and TGR5 were evaluated using digital image analysis. Results showed a significant decrease in S1PR2 expression (p=0.052) and a significant increase in TGR5 expression (p=0.02) in invasive CCA. Low S1PR2 expression was associated with reduced overall and disease-free survival rates (p<0.05), while TGR5 expression did not show significance (p=0.096). Multivariate analysis revealed low S1PR2 to be the only independent predictor of poor prognosis. Therefore, low S1PR2 expression may indicate a negative outcome in patients with resected extrahepatic CCA.
Pubmed:
37774230
DOI:
10.15403/jgld-4841
