mProX™ Human PTGER4 Stable Cell Line

Product Information

Target Protein

PTGER4

Target Family

Prostanoid Family

Target Protein Species

Human

Host Cell Type

Ishikawa; CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Cancer Research; Reproductive Research

Related Diseases

Basosquamous Carcinoma; Malignant Epithelial Mesothelioma

Gene ID

5734

UniProt ID

P35408

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Four distinct prostanoid E receptors (EP), referred to as PTGER1-4 (also known as EP1-4), which each have seven transmembrane-spanning domains, mediate the biological effects of PGE2. These receptors are coupled to a variety of intracellular signal transduction pathways, including those that cause cAMP increases (PTGER2 and PTGER4), decreases in cAMP (PTGER3), and Ca2+ mobilization (PTGER1). PTGER4 is the most frequently expressed EP subtype among the PTGERs, and it has been shown to have functional responses in B- and T-lymphocytes, eosinophils, monocytes, and macrophages. PGE2 has been linked to the activation of PTGER4 in a number of biological activities, including the control of immunological responses and cytokine synthesis. The customized PTGER4 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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I customized a PTGER4 cell line and the products definitely stand out. Jul 04 2023

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Creative biolabs can offer a good customized service. Apr 06 2023

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FAQ

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Published Data

Fig.1 PTGER4 enhances tumour growth.

In WT Ishikawa cells compared to Ishikawa cells stably transfected with the full length PTGER4 cDNA transcript, PTGER4 mRNA expression was observed.

Ref: Catalano, Rob D., et al. "Hypoxia and prostaglandin E receptor 4 signalling pathways synergise to promote endometrial adenocarcinoma cell proliferation and tumour growth." PloS one 6.5 (2011): e19209.

Pubmed: 21589857

DOI: 10.1371/journal.pone.0019209

Research Highlights

SHOX2 and PTGER4 methylation measurements in plasma DNA enabled LC identification and nonmalignant disease distinction. The creation of a diagnostic test based on this panel might be clinically useful when used in conjunction with current imaging methods to enhance risk classification for LC.
Weiss, Gunter, et al. "Validation of the SHOX2/PTGER4 DNA methylation marker panel for plasma-based discrimination between patients with malignant and nonmalignant lung disease." Journal of Thoracic Oncology 12.1 (2017): 77-84.
Pubmed: 27544059 DOI: 10.1016/j.jtho.2016.08.123

The intestinal stem cell niche and the repair of damaged epithelium depend on PTGER4+ intestinal macrophages. This discovery paves the door for the creation of a new class of therapeutic targets to support the healing capabilities of macrophages and favor remission in IBD patients.
Na, Yi Rang, et al. "Prostaglandin E2 receptor PTGER4-expressing macrophages promote intestinal epithelial barrier regeneration upon inflammation." Gut 70.12 (2021): 2249-2260.
Pubmed: 33558271 DOI: 10.1136/gutjnl-2020-322146