mProX™ Human P2RY6 Stable Cell Line

Product Information

Target Protein

P2RY6

Target Family

P2Y Family

Target Protein Species

Human

Host Cell Type

HT-29; CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Cancer Research; Cardiovascular Research

Related Diseases

Arthrogryposis; Infiltrative Basal Cell Carcinoma

Gene ID

5031

UniProt ID

Q15077

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Diphospho-nucleotides cause P2RY6 to become active. The endogenous ligands for P2RY6 include UDP and UTP, with UDP being around 100 times more powerful than UTP. P2RY6 is widely expressed at the tissue level in a variety of tissues, including the placenta, spleen, brain, thymus, heart, and blood vessels. P2RY6 is found in many different cell types, including immune cells, intestinal epithelial cells, vascular endothelial cells, and microglia, at the cellular level. The interaction of P2RY6 and the released UTP from injured cells might cause microglia to phagocytose. On the other hand, P2RY6 controls the release of interleukin-8 from macrophages and monocytes, which is crucial for controlling immunological inflammation. Therefore, targeting P2RY6 may be a potential approach to treating inflammatory, neurodegenerative, and illnesses associated with microglia activation. The customized P2RY6 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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Cost-effective and scalable bioprocess with robust and reliable tech transfer. Jul 19 2023

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FAQ

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Published Data

Fig.1 The invalidation of the P2ry6 gene modulates AKT signaling.

Mice treated with AOM-DSS according to the instructions or control animals left untreated had their distal colons isolated. Western blots were used to analyze the expression of -catenin, AKT, and AKT phosphorylation on Thr308. To guarantee comparable protein loading and lysate integrity, GAPDH expression was employed.

Ref: Placet, Morgane, et al. "The G protein-coupled P2Y6 receptor promotes colorectal cancer tumorigenesis by inhibiting apoptosis." Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 1864.5 (2018): 1539-1551.

Pubmed: 29454075

DOI: 10.1016/j.bbadis.2018.02.008

Research Highlights

Through the modification of the Hippo/YAP and Wnt/β-catenin signaling pathways, P2RY6 is a significant positive regulator of skin carcinogenesis.
Xu, Peng, et al. "P2RY6 has a critical role in mouse skin carcinogenesis by regulating the YAP and β-catenin signaling pathways." Journal of Investigative Dermatology 142.9 (2022): 2334-2342.
Pubmed: 35304248 DOI: 10.1016/j.jid.2022.02.017

This study provided fresh insight into the precise pro-inflammatory role that P2RY6-mediated signaling plays in neuroinflammation, which may open up new possibilities for the management of inflammatory reactions in the brain.
Timmerman, Raissa, Ella A. Zuiderwijk-Sick, and Jeffrey J. Bajramovic. "P2Y6 receptor-mediated signaling amplifies TLR-induced pro-inflammatory responses in microglia." Frontiers in Immunology 13 (2022): 967951.
Pubmed: 36203578 DOI: 10.3389/fimmu.2022.967951