mProX™ Human OXGR1 Stable Cell Line

Product Information

Target Protein

OXGR1

Target Family

α-Ketoglutarate Family

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

Inflammation Research

Related Diseases

Tympanic Membrane Disease; Bleeding Disorder

Gene ID

27199

UniProt ID

Q96P68

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

OXGR1, a G-protein coupled receptor (GPCR) expressed in cortical connecting tubule and collecting duct type B intercalated cells, encodes the 2-oxoglutarate (α-ketoglutarate [AKG]) receptor 1. OXGR1 mediates cellular Ca2+ uptake in response to its ligand AKG, a recognized secondary GPCR message. When the well-established calcium NL therapy, citrate, is consumed, AKG is produced and expelled through the kidneys. According to mouse model and perfused connecting tubule research, OXGR1 signals through PKC to enhance the pendrin chloride-bicarbonate exchanger upon binding to AKG. Both Slc26a4/pendrin−/− and Oxgr1−/− mice showed reduced urine alkalinization. Pendrin mutant mice also had urinary calcium squandering, which increases the chance of developing calcium stones, along with decreased expression of the apical TRPV5 and basolateral NCX1 in the cortical connecting tubule. The customized OXGR1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The customized OXGR1 stable cell line can be used in antibody discovery and development. Sep 22 2023

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The cell line can be used as model systems to investigate fundamental aspects of cell biology and biochemistry. Feb 04 2023

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FAQ

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Published Data

Fig.1 OXGR1 overexpression in cardiomyocytes inhibited phenylephrine-induced hypertrophy.

Adenovirus infection with human OXGR1 (Ad-OXGR1) results in the expression of OXGR1 in neonatal rat cardiomyocytes (NRCM), as shown by a Western blot study. Ad-LacZ, an adenovirus that expresses LacZ, was utilized as a control.

Ref: Omede, Ameh, et al. "The oxoglutarate receptor 1 (OXGR1) modulates pressure overload-induced cardiac hypertrophy in mice." Biochemical and biophysical research communications 479.4 (2016): 708-714.

Pubmed: 27693579

DOI: 10.1016/j.bbrc.2016.09.147

Research Highlights

Immune-responsive gene 1 (IRG1) is rapidly induced in myeloid lineage cells by pathogens and inflammatory conditions. The immunomodulatory metabolite itaconate (ITA) is produced by the aconitate decarboxylase (ACOD1) encoded by the IRG1 gene.
Zeng, Yi-Rong, et al. "The immunometabolite itaconate stimulates OXGR1 to promote mucociliary clearance during the pulmonary innate immune response." The Journal of Clinical Investigation 133.6 (2023).
Pubmed: 36919698 DOI: 10.1172/JCI160463

These results demonstrate a hitherto unrecognized mechanism for the beneficial effects of resistance training, utilizing systemically generated AKG as an adrenal stimulator of muscle hypertrophy and fat loss.
Yuan, Yexian, et al. "Exercise-induced α-ketoglutaric acid stimulates muscle hypertrophy and fat loss through OXGR1-dependent adrenal activation." The EMBO Journal 39.7 (2020): e103304.
Pubmed: 32104923 DOI: 10.15252/embj.2019103304