mProX™ Human MC4R Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
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Patrick Liam (Creative Biolabs Scientific Support)
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Patrick Liam (Creative Biolabs Scientific Support)
Published Data
Fig.1 The osteoblasts interact with α-MSH through the MC4R receptor, facilitating their functional response.
MC3T3-E1 cells, engineered with MC4R shRNA (−1/-2) or a scramble control shRNA ("Ctrl shRNA"), were exposed to α-MSH (10 nM) for 1 hour. Subsequently, these cells were subjected to treatment with dexamethasone ("Dex," 1 μM), or in combination with α-MSH (10 nM, pre-treated for 1 hour), for a duration of 48 hours, after which cellular viability was assessed.Experiments in this figure were repeated for four times. Data were presented as mean ± S.D. #p < 0.05.
Ref: Guo, Shiguang, et al. "α-Melanocyte stimulating hormone attenuates dexamethasone-induced osteoblast damages through activating melanocortin receptor 4-SphK1 signaling." Biochemical and biophysical research communications 469.2 (2016): 281-287.
Pubmed: 26631960
DOI: 10.1016/j.bbrc.2015.11.104
Research Highlights
de Lourdes Rabelo Guimaraes M, et al. "Evaluation of clinical and genetic factors in obstructive sleep apnoea.." Acta otorhinolaryngologica Italica : organo ufficiale della Societa italiana di , 2023.
The correlation between presumed candidate genes for obstructive sleep apnoea (OSA) and clinical OSA phenotypes was evaluated in this study. The researchers compared polysomnographic patterns, clinical data, morbidities, dental factors and genetic data for several polymorphisms in PER3, BDNF, NRXN3, APOE, HCRTR2, and MC4R between confirmed OSA cases and ethnically matched clinically unaffected controls in a case-control study. A logistic regression model was developed to predict OSA using the combined data. The results showed no significant association between the tested genetic variants and OSA phenotype. However, a predictive algorithm was developed that included gender, age, snoring, hypertension, mouth breathing, and one specific genetic marker, resulting in a 76.5% specificity and 71.6% sensitivity in diagnosing OSA. This model could potentially be applied in clinical settings for risk stratification for OSA, pending validation through larger prospective studies.
Pubmed:
37814975
DOI:
10.14639/0392-100X-N2532
Cao Q, et al. "Modular, automated synthesis of spirocyclic tetrahydronaphthyridines from primary ." Communications chemistry, 2023.
Spirocyclic tetrahydronaphthyridines (THNs) hold significant potential as scaffolds for drug discovery efforts. Yet, their valuable 3D chemical space remains largely unexplored due to limited and non-scalable synthetic methods. A team of researchers now presents an automated, continuous flow synthesis of alpha-alkylated and spirocyclic 1,2,3,4-tetrahydro-1,8-naphthyridines ("1,8-THNs"), as well as their regioisomeric 1,6-THN analogues. Using a photoredox-catalysed hydroaminoalkylation (HAA) approach in combination with intramolecular S(N)Ar N-arylation, they provide a highly modular access to the four isomeric THN cores from abundant primary amine feedstocks. Furthermore, they demonstrate the versatility of their novel methodology through a brief synthesis of Pfizer's MC4R antagonist PF-07258669.
Pubmed:
37794068
DOI:
10.1038/s42004-023-01012-2
