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  • mProX™ Human MC2R Stable Cell Line

    [CAT#: S01YF-0923-PY117]
    Product Category:
    Membrane Protein Stable Cell Lines
    Subcategory:
    GPCR Cell Lines

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    Based on this stable cell line, we also provide cell-based in vitro assays to evaluate the effects of your compounds or antibodies.

    Sub Cat Product Name Target Protein Species Host Cell Type Assay Types Inquiry Datasheet
    S01YF-1122-KX603 Magic™ Rat MC2R/MRAP in Vitro cAMP Assay Rat CHO-K1 cAMP Assay

    Product Information

    Target Protein
    MC2R
    Target Family
    Melanocortin Family
    Target Protein Species
    Mouse
    Host Cell Type
    3T3-L1;CHO-K1;HEK293
    Target Classification
    GPCR Cell Lines
    Target Research Area
    CNS Research;Metabolic Research
    Related Diseases
    Glucocorticoid Deficiency 1;Familial Glucocorticoid Deficiency
    Gene ID
    Mouse: 17200
    UniProt ID
    Mouse: Q64326

    Product Properties

    Biosafety Level
    Level 1
    Activity
    Yes
    Quantity
    10⁶ cells per vial
    Applications
    MC2R, or the Melanocortin 2 Receptor, is primarily known for its role in the adrenal axis. It is expressed in the adrenal cortex and is essential for the production of corticosteroids. Activation of MC2R by its ligand, ACTH, stimulates the production of cortisol, a critical hormone for stress response, metabolism, and immune function. Mutations in the MC2R gene can lead to familial glucocorticoid deficiency, a condition characterized by the lack of cortisol production. Understanding the function and regulation of MC2R is crucial for insights into adrenal biology, stress response, and potential treatments for adrenal insufficiencies.

    Protocols

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    FAQ

    chat Linda (Verified Customer)

    How does MC2R contribute to the response of bovine ovaries to stress? Feb 02 2023

    chat Patrick Liam (Creative Biolabs Scientific Support)

    MC2R/MRAP2 activation in bovine ovaries responds to ACTH as a consequence of stress, altering the expression of steroidogenic enzymes and suggesting that glucocorticoids could be produced locally. Feb 02 2023

    chat Rebecca (Verified Customer)

    What is the role of MC2R in familial glucocorticoid deficiency? Mar 21 2020

    chat Patrick Liam (Creative Biolabs Scientific Support)

    Variants in the MC2R gene can lead to familial glucocorticoid deficiency, as evidenced by a Chinese family with affected siblings having a homozygotic variant of c.712C>T/p.H238Y in MC2R, showing novel clinical features including congenital heart defects. Mar 21 2020

    Published Data

    Fig.1 Mc2r knockdown reduces lipid accumulation

    The relative quantity of extractable dye reveals diminished lipid content in the knockdown cell lines. Absorbance measurements were conducted at 530 nm, with subsequent data normalization relative to the control group.

    Ref: Betz, M. J., et al. "Mc2 receptor knockdown modulates differentiation and lipid composition in adipocytes." Hormone and metabolic research 44.09 (2012): 670-675.

    Pubmed: 22723268

    DOI: 10.1055/s-0032-1314854

    Research Highlights

    Duan Y, et al. "Genetic and phenotypic spectrum of non-21-hydroxylase-deficiency primary adrenal ." Journal of medical genetics, 2023.
    Primary adrenal insufficiency (PAI) is a rare and life-threatening condition that requires a thorough understanding of its numerous potential causes for proper diagnosis. The genetic makeup and manifestations of PAI were retrospectively studied in a group of 111 patients with non-21OHD, divided into four groups according to underlying pathogenesis. Mutations in several genes were identified, with STAR and NR0B1 being the most prevalent. Common clinical presentations included hyperpigmentation, dehydration, vomiting, and abnormal external genitalia. It was found that age of onset and clinical characteristics differed based on the specific cause of PAI. A flowchart was developed to aid in diagnosing PAI using specific clinical features and molecular tests, leading to accurate diagnosis in 84.8% of patients before next-generation DNA sequencing.
    Pubmed: 37586839   DOI: 10.1136/jmg-2022-108952

    Shaughnessy CA, et al. "Functional characterization of melanocortin 2 receptor (Mc2r) from a lobe-finned ." General and comparative endocrinology, 2023.
    Recent studies by researchers from our group have shed light on the function of melanocortin 2 receptors (Mc2r) in basal families of actinopterygians. These studies have revealed that even in the most primitive ray-finned fishes, Mc2r is dependent on the presence of Mrap1 and has a preference for adrenocorticotrophic hormone (ACTH). However, no studies have been conducted on the Mc2r function in basal sarcopterygians, specifically lobe-finned fishes such as coelacanths and lungfishes. To address this gap, the team has conducted the first molecular and functional characterization of an Mc2r from a lobe-finned fish, the West African lungfish (Protopterus annectens). Through experiments with mammalian and zebrafish cell lines, it was found that the lungfish Mc2r requires co-expression with the lungfish Mrap1 and has a strong preference for ACTH over other related hormones, such as alpha-melanocyte stimulating hormone (α-MSH). This preference was confirmed by multiple-alanine substitutions targeting specific motifs within the Mc2r protein. These findings indicate that the function of Mc2r differs significantly between bony vertebrates and cartilaginous fishes, with the latter exhibiting a unique and rapid evolution of Mc2r function. These conclusions are further supported by a molecular clock analysis, which shows a high rate of sequence divergence in the Mc2r of different vertebrate groups. While these findings provide important insights into the molecular evolution of Mc2r, further research is needed to fully understand the mechanisms underlying Mrap1 dependence and ACTH selectivity.
    Pubmed: 37562700   DOI: 10.1016/j.ygcen.2023.114356

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR CLINICAL PROCEDURES" For licensing inquiries, please contact
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