mProX™ Human LHCGR Stable Cell Line
- Product Category:
- Membrane Protein Stable Cell Lines
- Subcategory:
- GPCR Cell Lines
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Published Data
Fig.1 The activation of ERK1/2 phosphorylation in response to various concentrations of distinct hCG variants was examined.
HEK293 cells, bearing stable LHCGR expression, underwent stimulation using hCG enriched with CGA, containing either CGB7 or CGB3/5/8. Western blot analysis (n = 3 independent trials; sample blots depicted) was employed to assess levels of phosphorylated ERK1/2 (P-ERK1/2), total ERK1/2, and GAPDH proteins post 10-minute hCG exposure, with single subunits and recombinant LH as reference standards. The blot was initially probed with anti-P-ERK1/2 (upper plot), subsequently stripped and incubated with anti-ERK1/2 and GAPDH antibodies (lower plot).
Ref: Biskup, Karina, et al. "N-and O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit." Glycoconjugate Jou
Pubmed: 32767150
DOI: 10.1007/s10719-020-09936-w
Research Highlights
Shan Y, et al. "Arbutin inhibits androgen biosynthesis by rat immature Leydig cells in vitro.." Reproductive toxicology (Elmsford, N.Y.), 2023.
In this study, the effects of arbutin on Leydig cell function were investigated through an in vitro model. The impact on androgen levels and gene and protein expression related to Leydig cell steroidogenesis was measured in rat immature Leydig cells. Exposure to arbutin at concentrations of 0.5-50 muM for 3 hours resulted in a significant inhibition of androgen secretion. Additionally, at a concentration of 50muM, arbutin blocked the stimulatory effects of luteinizing hormone and 8Br-cAMP on androgen secretion. The analysis also showed a downregulation of key genes involved in androgen production. Furthermore, computer program analysis predicted potential toxicity, including hepatoxicity, due to the absorption rate and elimination half-life of arbutin. Therefore, the results suggest that arbutin may negatively affect male reproductive health.
Pubmed:
37783241
DOI:
10.1016/j.reprotox.2023.108476
de la Fuente A, et al. "Transcriptome Signature of Immature and In Vitro-Matured Equine Cumulus-Oocytes ." International journal of molecular sciences, 2023.
In this study, immature and in vitro-matured (MII) oocytes (OC) and cumulus cells (CC) were explored through RNA extraction, library preparation, and RNA sequencing. 13,918 gene transcripts were identified in OC, with 538 differentially expressed genes (DEG) between immature OC and in vitro-matured OC. In CC, 13,104 genes were expressed with 871 DEG. Further analysis revealed that these differentially expressed genes were involved in pathways related to nuclear maturation in OC and GTPase activity, extracellular matrix organization, and collagen trimers in CC. Interestingly, the follicle-stimulating hormone receptor gene (FSHR) and luteinizing hormone/choriogonadotropin receptor gene (LHCGR) showed differential expressions between CC-MII and immature CC samples. These findings provide a basis for further research into biological pathways related to oocyte maturation in horses, with potential implications for improving assisted reproductive technologies (ART) in equines.
Pubmed:
37762020
DOI:
10.3390/ijms241813718
