mProX™ Human KIT Stable Cell Line

Product Information

Target Protein

KIT

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research; Digestive and Renal Research; Reproductive Research

Related Diseases

Piebald Trait and Gastrointestinal Stromal Tumor. Among its related pathways are Apoptotic Pathways in Synovial Fibroblasts and Dasatinib-resistant KIT mutants

Gene ID

3815

UniProt ID

P10721

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The KIT gene codes for the production of a member of the receptor tyrosine kinase protein family. A process known as signal transduction is used by receptor tyrosine kinases to transfer messages from the cell surface within the cell. The KIT protein is located in the membrane of some types of cells where it binds to a particular protein known as stem cell factor. The KIT protein is activated by this interaction, and it adds a cluster of oxygen and phosphorus atoms at certain locations to other proteins within the cell to activate them as well. Phosphorylation is the process that causes several proteins in various signaling pathways to become active. The customized KIT stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Kevin

I think this is a quality KIT overexpression cell line for drug discovery. Jul 18 2021

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chat Anthony

Very good KIT cell line for drug discovery. Feb 12 2020

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FAQ

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Published Data

Fig.1 Expression of c-kit mRNA in HD and ALCL cell lines.

All HD and ALCL cell lines tested negative for c-kit RNA using RT-PCR and a set of primers we generated. Using a c-kit-specific probe for hybridization and southern blot transfer, neither HD nor ALCL cell lines showed any bands.

Ref: Rassidakis, George Z., et al. "Lack of c-kit (CD117) expression in CD30+ lymphomas and lymphomatoid papulosis." Modern pathology 17.8 (2004): 946-953.

Pubmed: 15105813

DOI: 10.1038/modpathol.3800144

Research Highlights

Encoding a receptor tyrosine kinase (RTK) that reacts to stem cell factor (SCF), c-kit is a classical proto-oncogene. Involved in various physiological processes such as hematopoiesis, gastrointestinal motility, and pigmentation, C-KIT signaling is a crucial regulator of cell proliferation, survival, and migration.
Sheikh, Emana, et al. "Role and significance of c-KIT receptor tyrosine kinase in cancer: A review." Bosnian Journal of Basic Medical Sciences 22.5 (2022): 683.
Pubmed: 35490363 DOI: 10.17305/bjbms.2021.7399

The feline v-kit sarcoma oncogene's discovery led to the discovery of the Kit proto-oncogene. The Kit ligand that causes Kit dimerization and activation is called stem cell factor (SCF).
Roskoski Jr, Robert. "The role of small molecule Kit protein-tyrosine kinase inhibitors in the treatment of neoplastic disorders." Pharmacological research 133 (2018): 35-52.
Pubmed: 29704617 DOI: 10.1016/j.phrs.2018.04.020