mProX™ Human KISS1R Stable Cell Line

Wang Y, et al. "Clinical and molecular features of 40 Chinese patients with idiopathic ." Translational andrology and urology, 2023.
Male idiopathic hypogonadotropic hypogonadism (IHH) is a rare and heterogeneous genetic disorder characterized by reduced production of testosterone and sperm, resulting in delayed or absent puberty and infertility. It can be divided into two forms: Kallmann syndrome (KS) and olfactory normal IHH (nIHH). However, approximately half of the genetic causes and associated mechanisms of IHH are still unknown. To further elucidate the genetic basis of IHH, a retrospective study was conducted on 40 IHH patients, including 22 with KS and 18 with nIHH. Whole exome sequencing (WES) and Sanger sequencing were used to identify genetic mutations in the proband genomic DNA (gDNA). Results revealed ten new genetic mutations related to IHH in both familial and sporadic cases, with a detection rate of 30% among the patients. The most common mutations were found in FGFR1 (7.5%), followed by ANOS1, CHD7, and KISS1R (5% each), and SEMA3E, PROKR2, and SOX10 (2.5% each). All missense and nonsense mutations were predicted to have harmful or pathogenic effects by software analysis, and were highly conserved among different species and over evolution. These findings expand the current understanding of the genetic profile of IHH and may aid in the diagnosis, treatment, and genetic counseling of affected individuals.
Pubmed: 37814704   DOI: 10.21037/tau-23-225

Santos LC, et al. "Kisspeptin treatment reverses high prolactin levels and improves gonadal function ." Scientific reports, 2023.
The effects of kisspeptin-10 (Kp10) on restoring gonadal function in hypothyroid male rats were investigated in this study. Hypothyroidism was induced through the administration of 6-propyl-2-thiouracil (PTU) for three months. In the last month, half of the hypothyroid rats were treated with Kp10. Results showed that hypothyroidism led to a decrease in testicular and sex gland mass, reduced proliferation of the seminiferous epithelium, and impaired sperm morphology, motility, and vigor. It also caused a decrease in plasma luteinizing hormone (LH) and testosterone levels and an increase in prolactin secretion. This condition also reduced the expression of Kiss1 and Kiss1r protein and gene in the testis, as well as Star and Cyp11a1 mRNA levels. In the pituitary, hypothyroidism reduced Lhb, Prl, and Drd2 expression while increasing Tshb and Gnrhr levels. In the hypothalamus, it increased Pdyn and Kiss1r expression and reduced Gnrh1. Treatment with Kp10 in hypothyroid rats restored testicular and seminal vesicle morphology, improved sperm morphology and motility, reversed high prolactin levels, and increased LH and testosterone levels. Furthermore, Kp10 treatment led to an increase in testicular expression of Kiss1, Kiss1r, Fshr, and Nr5a1, and pituitary expression of Kiss1. These findings highlight the inhibitory effects of hypothyroidism on the male gonadal axis and sperm quality, and demonstrate the potential of Kp10 to reverse these effects and improve gonadal function and sperm quality in hypothyroid rat models.
Pubmed: 37798396   DOI: 10.1038/s41598-023-44056-z