mProX™ Human KDR Stable Cell Line

Product Information

Target Protein

KDR

Target Family

Kinases/Enzyme Drug Discovery Assays and Products

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Kinase Cell Lines

Target Research Area

Cancer Research

Related Diseases

Hemangioma, Capillary Infantile and Angioma, Tufted. Among its related pathways are Apoptotic Pathways in Synovial Fibroblasts and GPCR Pathway

Gene ID

3791

UniProt ID

P35968

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Another name for the type IV receptor tyrosine kinase, kinase insert domain receptor (KDR), is vascular endothelial growth factor receptor 2 (VEGFR-2). It is encoded by the human gene KDR. KDR is now referred to as CD309, or the cluster of differentiation 309. Fetal Liver Kinase 1 (Flk1) is another name for KDR. The Q472H germline KDR genetic variant has been linked to microvessel density in non-small cell lung cancer (NSCLC) and has been found to impact VEGFR-2 phosphorylation. It has been demonstrated that the kinase insert domain receptor interacts with SHC1, Annexin A5, and SHC2. The customized KDR stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Thomas

The human KDR cell line is an essential tool for anyone studying drug discovery. Jul 28 2022

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chat Matthew

The KDR overexpression cell line is very good to use and is of high quality and safety. Mar 03 2021

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FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 Inhibition of KDR phosphorylation in 293/KDR cell line.

Before being stimulated with rhVEGF (10 ng/ml), cells were cultured in 6-well plates and treated with the inhibitor as directed for 30 minutes. Representative Western blot with 293/KDR cell lysates that were probed with particular anti-phospho-and anti-total KDR after being solved in SDS-PAGE and transferred to PVDF membranes.

Ref: Vicari, Daniele, et al. "Engineered conformation-dependent VEGF peptide mimics are effective in inhibiting VEGF signaling pathways." Journal of Biological Chemistry 286.15 (2011): 13612-13625.

Pubmed: 21321115

DOI: 10.1074/jbc.M110.216812

Research Highlights

Angiogenesis is the process by which new blood vessels form from the existing vascular. It is a physiological process that is necessary for cell growth, tissue repair, wound healing, and the female reproductive system. Diseases like cancer, diabetes, atherosclerosis, rheumatism, and arthritis are associated with the regulation of angiogenesis.
Modi, Siddharth J., and Vithal M. Kulkarni. "Vascular endothelial growth factor receptor (VEGFR-2)/KDR inhibitors: medicinal chemistry perspective." Medicine in Drug Discovery 2 (2019): 100009.

The knockdown resistance (kdr) that results in resistance to pyrethroids and DDT is one of the most extensively studied and studied processes. The voltage-sensitive sodium channel, which is involved in the initiation and propagation of action potentials in nerve cells, is thought to be the mechanism.
Auteri, Michelangelo, et al. "Insecticide resistance associated with kdr mutations in Aedes albopictus: an update on worldwide evidences." BioMed research international 2018 (2018).
Pubmed: 30175124 DOI: 10.1155/2018/3098575