mProX™ Human KCNN2 Stable Cell Line

Product Information

Target Protein

KCNN2

Target Family

SKCa

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Related Diseases

Dystonia; Myoclonic; Neurodevelopmental Disorder

Gene ID

3781

UniProt ID

Q9H2S1

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

Another name for the ion channel protein KCNN2 is KCa2.2. In vertebrate neurons, action potentials are followed by an afterhyperpolarization (AHP) that can last for a few seconds and has a significant impact on the neuron's firing pattern. Different calcium-activated potassium channels mediate each of the AHP's kinetically unique components. The slow component of synaptic AHP is assumed to be regulated by the KCa2.2 protein, which is activated prior to membrane hyperpolarization. Together with three other calmodulin-binding subunits, KCa2.2, an integral membrane protein, forms a voltage-independent calcium-activated channel. This protein belongs to the family of potassium channels that are activated by calcium. For the KCNN2 gene, two transcript variants encoding distinct isoforms have been discovered. The customized KCNN2 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

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Published Data

Fig.1 Increase of Kcnn2-expressing neurons in M1 in PAe mice.

Analysis of Kcnn2 immunohistochemistry at P30. In comparison to control (PBS-exposed) mice, PAE animals had more Kcnn2 + cells (arrowheads in a). In Hsf1 KO mice, PAE does not cause an increase in Kcnn2 + cells.

Ref: Mohammad, Shahid, et al. "Kcnn2 blockade reverses learning deficits in a mouse model of fetal alcohol spectrum disorders." Nature neuroscience 23.4 (2020): 533-543.

Pubmed: 32203497

DOI: 10.1038/s41593-020-0592-z

Research Highlights

In AF exacerbated by HF, the expression of KCNN2/3 and HDAC2 is reduced. In atrial cells, Kcnn3 mRNA levels are directly regulated by Hdac2. More research is needed to confirm the mechanistic and therapeutic relevance of epigenetic electrophysiological effects in AF.
Rahm, Ann-Kathrin, et al. "HDAC2-dependent remodeling of KCa2. 2 (KCNN2) and KCa2. 3 (KCNN3) K+ channels in atrial fibrillation with concomitant heart failure." Life Sciences 266 (2021): 118892.
Pubmed: 33310041 DOI: 10.1016/j.lfs.2020.118892

The calcium-activated, small conductance potassium channel 2 (SK2) is encoded by KCNN2. Unknown human illnesses are linked to KCNN2 variations, while rodent models with spontaneous Kcnn2 mutations exhibit aberrant gait and locomotor activity, tremor, and memory impairments.
Mochel, Fanny, et al. "Variants in the SK2 channel gene (KCNN2) lead to dominant neurodevelopmental movement disorders." Brain 143.12 (2020): 3564-3573.
Pubmed: 33242881 DOI: 10.1093/brain/awaa346