mProX™ Human KCNN1 Stable Cell Line

Product Information

Target Protein

KCNN1

Target Family

SKCa

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Related Diseases

Mental Retardation; Developmental And Epileptic Encephalopathy

Gene ID

3780

UniProt ID

Q92952

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

A human gene that codes for the KCa2.1 protein is called potassium intermediate/small conductance calcium-activated channel, subfamily N, member 1. It is sometimes referred to as KCNN1. In vertebrate neurons, action potentials are followed by an afterhyperpolarization (AHP) that can last for a few seconds and has a significant impact on the neuron's firing pattern. Different calcium-activated potassium channels mediate each of the AHP's kinetically unique components. The protein that this gene encodes is hypothesized to control neuronal excitability by contributing to the slow part of synaptic AHP. It is activated prior to membrane hyperpolarization. Together with three other calmodulin-binding subunits, the integral membrane protein KCa2.1 forms a voltage-independent calcium-activated channel. The KCNN1 gene belongs to the potassium KCNN family. The customized KCNN1 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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The Human KCNN1 Stable Cell Line is so great! Jun 28 2022

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Success assurance with the best cell line, right from the start. Apr 04 2023

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FAQ

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Published Data

Fig.1 Effect of acacetin on SKCa1 channel stably expressed in HEK 293 cells.

In an exemplar cell that expressed human KCNN1, SKCa1 current was triggered by 200-ms step voltages ranging from -70 to +80 mV from a holding potential of -80 mV both in the presence and absence of 10 μM acacetin.

Ref: Chen, Kui-Hao, et al. "The natural flavone acacetin blocks small conductance Ca2+-activated K+ channels stably expressed in HEK 293 cells." Frontiers in pharmacology 8 (2017): 716.

Pubmed: 29081746

DOI: 10.3389/fphar.2017.00716

Research Highlights

A rare and extremely aggressive bone tumor that primarily affects children and adolescents is called Ewing sarcoma (EwS). Osteolytic events in EwS seriously disrupt Ca2+ homeostasis, which is physiologically centered on the bone.
Fuest, Sebastian, et al. "Relevance of Abnormal KCNN1 Expression and Osmotic Hypersensitivity in Ewing Sarcoma." Cancers 14.19 (2022): 4819.
Pubmed: 36230742 DOI: 10.3390/cancers14194819

Refractory neuropathic pain is a result of injury to the peripheral somatosensory nervous system. Misfolded alterations in the expression of genes in primary sensory neurons are thought to be the biochemical cause of this illness.
Wang, Bing, et al. "A sensory neuron-specific long non-coding RNA reduces neuropathic pain by rescuing KCNN1 expression." Brain (2023): awad110.
Pubmed: 37012681 DOI: 10.1093/brain/awad110