mProX™ Human KCNK3 Stable Cell Line

Product Information

Target Protein

KCNK3

Target Family

TASK

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Related Diseases

Pulmonary Hypertension

Gene ID

3777

UniProt ID

O14649

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The KCNK3 gene in humans encodes a protein known as potassium channel subfamily K member 3. One of the potassium channel proteins in the superfamily with two pore-forming P domains, K2P3.1, is encoded by this gene. Extracellular acidification inhibits K2P3.1, an outwardly rectifying channel that is sensitive to variations in extracellular pH. It is also known as an acid-sensitive potassium channel, and isoflurane and halothane are anesthetics that activate it. There is currently no sequencing available to confirm transcript variations for this gene, despite the fact that three transcripts are found in northern blots. The customized KCNK3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Dorothy

I was very pleased with the QCs of this cell line. Jul 23 2022

chat Verified Customer

chat Joseph

The cell line worked very well. Apr 27 2021

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 DDSA mutations produce a gain-of-function phenotype in TASK-1.

Typical TEVC recordings of WT TASK-1 and DDSA mutant currents in response to 20 mV voltage steps from a holding potential of -80 mV to +50 mV.

Ref: Sörmann, Janina, et al. "Gain-of-function mutations in KCNK3 cause a developmental disorder with sleep apnea." Nature Genetics 54.10 (2022): 1534-1543.

Pubmed: 36195757

DOI: 10.1038/s41588-022-01185-x

Research Highlights

The two-pore domain potassium channel family includes TWIK-related acid-sensitive potassium channel 1 (TASK-1 encoded by KCNK3). This gene subfamily has been specifically connected to the human pulmonary circulation and is constitutively active in excitable cells, such as smooth muscle cells, at physiological resting membrane potentials.
Olschewski, Andrea, et al. "TASK-1 (KCNK3) channels in the lung: from cell biology to clinical implications." European Respiratory Journal 50.5 (2017).
Pubmed: 29122916 DOI: 10.1183/13993003.00754-2017

KCNK3 expression and activity in pulmonary artery smooth muscle cells and endothelial cells are markedly decreased in PAH and experimental PH. Increased proliferation, vasoconstriction, and inflammation were all encouraged by KCNK3 inhibition. Monocrotaline-induced PH was lessened when KCNK3 was pharmacologically activated in vivo, indicating that KCNK3 depletion is a critical step in the genesis of PAH and may therefore be addressed therapeutically.
Antigny, Fabrice, et al. "Potassium channel subfamily K member 3 (KCNK3) contributes to the development of pulmonary arterial hypertension." Circulation 133.14 (2016): 1371-1385.
Pubmed: 26912814 DOI: 10.1161/CIRCULATIONAHA.115.020951