mProX™ Human KCNJ3 Stable Cell Line

Product Information

Target Protein

KCNJ3

Target Family

GIRK

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Related Diseases

Epilepsy; Vitreoretinal Degeneration

Gene ID

3760

UniProt ID

P48549

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The human gene KCNJ3 encodes G protein-activated inward rectifier potassium channel 1 (GIRK-1). The majority of mammalian cells include potassium channels, which are involved in a variety of physiological reactions. This gene codes for a potassium channel of the inward-rectifier type and an integral membrane protein. The encoded protein is regulated by G-proteins and is involved in heartbeat regulation. It has a stronger propensity to permit potassium to flow into a cell rather than out of a cell. It joins forces with three additional potassium channels that are activated by G proteins to form a hetero-tetrameric pore-forming complex. The customized KCNJ3 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Matthew

I was very pleased with the QCs of the KCNJ3 cell line. Mar 10 2020

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chat Cynthia

They are an excellent investment because of their unparalleled performance and quality. Sep 26 2020

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FAQ

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Published Data

Fig.1 Electrophysiological analysis of the Kir3.1-N83H mutant.

Recordings made on the Kir3.1-WT and Kir3.1-N83H channels in isolation. In HEK293T cells, Kir3.4 was used to express either Kir3.1-WT or Kir3.1-N83H. Initially, the cell currents were recorded in the cell-attached patch-clamp configuration (CAP; top) with the cells at a potential of -100 mV. The patches were removed to create the inside-out patch configuration (I/O; bottom) after the recording.

Ref: Yamada, Noriaki, et al. "Mutant KCNJ3 and KCNJ5 potassium channels as novel molecular targets in bradyarrhythmias and atrial fibrillation." Circulation 139.18 (2019): 2157-2169.

Pubmed: 30764634

DOI: 10.1161/CIRCULATIONAHA.118.036761

Research Highlights

In patients with breast cancer, overexpression of KCNJ3 in the main tumor has been linked to shorter survival periods and higher lymph node metastases.
Rezania, Simin, et al. "Overexpression of KCNJ3 gene splice variants affects vital parameters of the malignant breast cancer cell line MCF-7 in an opposing manner." BMC cancer 16 (2016): 1-15.
Pubmed: 27519272 DOI: 10.1186/s12885-016-2664-8

Several investigations have demonstrated that certain cancer types express ion channels abnormally. Among these, it has been found that the potassium channel gene KCNJ3 is increased in breast cancer patient tumors and correlates with positive lymph node status.
Kammerer, Sarah, et al. "KCNJ3 is a new independent prognostic marker for estrogen receptor positive breast cancer patients." Oncotarget 7.51 (2016): 84705.
Pubmed: 27835900 DOI: 10.18632/oncotarget.13224