mProX™ Human KCNJ11 Stable Cell Line

Product Information

Target Protein

KCNJ11

Target Family

KATP

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Diabetes Research

Related Diseases

Hyperinsulinemic Hypoglycemia; Diabetes Mellitus

Gene ID

3767

UniProt ID

Q14654

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The ATP-sensitive potassium (K-ATP) channel's subunits are made according to instructions provided by the KCNJ11 gene. K-ATP channels are made up of eight subunits each. The KCNJ11 gene produces four subunits, while the ABCC8 gene produces four more subunits. Beta cells are the pancreatic cells that secrete the hormone insulin, and they contain K-ATP channels. The amount of glucose in the bloodstream causes the K-ATP channels, which are embedded in cell membranes, to open and close. Simple sugar glucose serves as the body's main source of energy for the majority of its cells. When glucose levels rise, the K-ATP channels close, causing beta cells to release more insulin into the bloodstream and assisting in blood glucose regulation. The customized KCNJ11 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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I purchased the cell line and it work well for my research. Mar 02 2021

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I was so pleased with the cell line I received from this business. Oct 11 2021

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FAQ

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Published Data

Fig.1 Specific deletion of the Kcnj11 gene in β cells (βKcnj11-/-) severely impairs glucose tolerance and GIIS in mice.

The electrophysiology of Kcnj11fl/fl and βKcnj11-/- mice's β cells. On the left, you can see an example of whole-cell KATP current recordings in primary β cells that were separated from Kcnj11fl/fl and βKcnj11-/- mice. Conductance adjusted to cell capacitance is in the middle. The perforated patch was used to evaluate the resting membrane potential of primary β cells from both control and βKcnj11-/- mice.

Ref: Oduori, Okechi S., et al. "Gs/Gq signaling switch in β cells defines incretin effectiveness in diabetes." The Journal of clinical investigation 130.12 (2020): 6639-6655.

Pubmed: 33196462

DOI: 10.1172/JCI140046

Research Highlights

In pancreatic beta cells, the ATP-sensitive potassium (KATP) channel mediates insulin secretion. This channel is a heteromeric protein made up of sulfonylurea receptor 1 subunits surrounding the pore and four inward-rectifier potassium ion channel (Kir6.2) tetramers, which constitute the KATP channel's pore.
Haghvirdizadeh, Polin, et al. "KCNJ11: genetic polymorphisms and risk of diabetes mellitus." Journal of diabetes research 2015 (2015).
Pubmed: 26448950 DOI: 10.1155/2015/908152

Research has demonstrated a correlation between mutations in the KCNJ11 gene and the following conditions: hyperinsulinemic hypoglycemia, type 2 diabetes mellitus (T2DM), maturity-onset diabetes of the young 13 (MODY13), and newborn diabetes mellitus (NDM).
He, Binbin, Xia Li, and Zhiguang Zhou. "Continuous spectrum of glucose dysmetabolism due to the KCNJ11 gene mutation-Case reports and review of the literature." Journal of Diabetes 13.1 (2021): 19-32.
Pubmed: 32935446 DOI: 10.1111/1753-0407.13114