mProX™ Human HTR6 Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types
S01YF-1122-KX934	Magic™ Mouse HTR6 in Vitro cAMP Assay	Mouse	1321N1	cAMP Assay
S01YF-1122-KX935	Magic™ Mouse HTR6 in Vitro Radioligand Binding Assay	Mouse	CHO-K1	Radioligand Binding Assay
S01YF-1122-KX936	Magic™ Rat HTR6 in Vitro cAMP Assay	Rat	CHO-K1	cAMP Assay

Product Information

Target Protein

HTR6

Target Family

5-Hydroxytryptamine Family

Target Protein Species

Human

Host Cell Type

IMCD3; CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

CNS Research

Related Diseases

Schizophrenia; Meckel Syndrome

Gene ID

3362

UniProt ID

P50406

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

One of the several 5-hydroxytryptamine (serotonin) receptors is HTR6. By interacting with serotonin, the active receptor encourages adenylate cyclase, which activates the cyclic AMP-dependent signaling cascade. According to reports, the receptor controls cholinergic neuronal transmission in the brain and is linked to neurological conditions like epilepsy, depression, schizophrenia, and Alzheimer's disease. Additionally, the majority of research have shown that inhibiting HTR6 can lessen the cognitive, working memory, and behavioral flexibility deficits associated with Alzheimer's disease. Therefore, using HTR6 as a promising therapeutic target for the treatment of neurological diseases is possible. Recently, more and more HTR6-targeting antidepressants and antipsychotic medications have been produced for the treatment of neurological disorders. The customized HTR6 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Mick (Verified Customer)

What is the mProX™ Human HTR6 Stable Cell Line? Aug 16 2022

chat Sherry Smith (Creative Biolabs Scientific Support)

The HTR6 stable cell line is a customized cell line. This cell line can be engineered to overexpress, knock out (KO), or knock down (KD). Aug 16 2022

chat Arnold (Verified Customer)

What host cells are typically used? Mar 06 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

HEK293 or CHO-K1 are used for this stable cell line. Mar 06 2023

Published Data

Fig.1 HTR6 ciliary targeting requires the ciliary trafficking adapter TULP3.

Serum-starved to encourage ciliogenesis, mouse TULP3 siRNAs (siTULP3 #1 or siTULP3 #2) or firefly luciferase (siLUC) as a negative control were transiently transfected into HTR6-IMCD3 cells. The cells were then immunostained with anti-ARL13B (green) and anti-HTR6 (red) antibodies.

Ref: Barbeito, Pablo, et al. "HTR6 and SSTR3 ciliary targeting relies on both IC3 loops and C-terminal tails." Life Science Alliance 4.3 (2021).

Pubmed: 33372037

DOI: 10.26508/lsa.202000746

Research Highlights

The vestibular function test indices and the level of 5-HT can both be used as reliable markers for VM observation, and the polymorphism of the 5-HTR6 rs770963777 site is associated with the start of VM.
Wu, Xia, et al. "Correlation of 5-HTR6 gene polymorphism with vestibular migraine." Journal of Clinical Laboratory Analysis 34.2 (2020): e23042.
Pubmed: 31587366 DOI: 10.1002/jcla.23042

Hippocampal neurons may use the HTR6-mediated mTORC1 pathway as a nutrition sensor to link memory function to dietary consumption.
Teng, Ling-Ling, et al. "Serotonin receptor HTR6-mediated mTORC1 signaling regulates dietary restriction-induced memory enhancement." PLoS Biology 17.3 (2019): e2007097.
Pubmed: 30883547 DOI: 10.1371/journal.pbio.2007097