mProX™ Human HTR2A Stable Cell Line

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types
S01YF-1122-KX916	Magic™ Rabbit HTR2A in Vitro IP1 Assay	Rabbit	CHO-K1	IP1 Assay
S01YF-1122-KX917	Magic™ Rabbit HTR2A in Vitro Radioligand Binding Assay	Rabbit	CHO-K1	Radioligand Binding Assay
S01YF-1122-KX918	Magic™ Rat HTR2A in Vitro Calcium Flux Assay	Rat	CHO-K1-Gα16	Calcium Flux Assay
S01YF-1122-KX919	Magic™ Rat HTR2A in Vitro IP1 Assay	Rat	CHO-K1	IP1 Assay
S01YF-1122-KX920	Magic™ Rat HTR2A in Vitro Radioligand Binding Assay	Rat	CHO-K1	Radioligand Binding Assay

Product Information

Target Protein

HTR2A

Target Family

5-Hydroxytryptamine Family

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

CNS Research; Cardiovascular Research; Digestive and Renal Research

Related Diseases

Major Depressive Disorder; Obsessive-Compulsive Disorder

Gene ID

3356

UniProt ID

P28223

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The neurotransmitter serotonin has a receptor in the body called HTR2A. It promotes the activation of alternative signaling pathways while suppressing G protein-mediated signaling. The central nervous system's 5-HT2A is activated, which raises body temperature and boosts ACTH release. 5-HT2A mediates the contractile reactions of vascular smooth muscle, as well as a part of the gastrointestinal smooth muscle, to 5-HT in the periphery. Additionally, it might contribute to arterial vasoconstriction. Phospholipase C is activated by 5-HT2A signaling, as is a second messenger pathway involving phosphatidylinositol and calcium that controls the activity of phosphatidylinositol 3-kinase and facilitates the release of Ca2+ ions from intracellular storage. It also affects neuronal activity, perception, cognition, and emotion, making it a potential drug and psychoactive substance receptor. The customized HTR2A stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Rae (Verified Customer)

What does the report look like? Feb 17 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

The report consists of a description of the method, the QC of flow cytometry to verify the surface expression at the protein level, and qPCR to verify the engineering results at the mRNA level. Feb 17 2023

chat Natalie (Verified Customer)

Which selection marker should I use? Jun 09 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

Creative Biolabs offers a variety of selection markers, such as puromycin (Puro), neomycin (Neo), hygromycin B (Hygro) and blasticidin (Bsd). Jun 09 2023

Published Data

Fig.1 The enhanced expression of inflammatory genes and lipogenesis were both influenced by the HTR2A/PPARg2 signaling pathway.

Pparg, Pparg1, and Pparg2 messenger RNA (mRNA) expression in the liver of SD rats. The liver of SD rats expressed Fas mRNA. PPARg2 protein expression in SD rats' liver and BRL-3A cells. Plin2 and Cd36's mRNA expression in BRL-3A.

Ref: Wang, Lulu, et al. "Gut-derived serotonin contributes to the progression of non-alcoholic steatohepatitis via the liver HTR2A/PPARγ2 pathway." Frontiers in Pharmacology 11 (2020): 553.

Pubmed: 32477107

DOI: 10.3389/fphar.2020.00553

Research Highlights

Serotonin 2A receptor remains a key pharmacological target for psychiatric, neurological, and cardiovascular diseases despite being heavily implicated in genetic association research. The architecture of the serotonin 2A receptor gene (HTR2A) was redefined by RNA sequencing, which also revealed novel mRNA transcript isoforms that made use of the gene's untranslated regions.
Ruble, Cara L., et al. "Genomic structure and expression of the human serotonin 2A receptor gene (HTR2A) locus: identification of novel HTR2A and antisense (HTR2A-AS1) exons." BMC genetics 17 (2016): 1-15.
Pubmed: 26738766 DOI: 10.1186/s12863-015-0325-6

The current study confirmed the association between HTR2A mutations and MDD treatment responsiveness to antidepressants, but not MDD susceptibility.
Kao, Chung-Feng, et al. "Gene-based association analysis suggests association of htr2a with antidepressant treatment response in depressed patients." Frontiers in Pharmacology 11 (2020): 1762.
Pubmed: 33519430 DOI: 10.3389/fphar.2020.559601