mProX™ Human HTR1A Stable Cell Line

Based on this stable cell line, we also provide cell-based in vitro assays to evaluate the effects of your compounds or antibodies.

Sub Cat	Product Name	Target Protein Species	Host Cell Type	Assay Types
S01YF-1122-KX865	Magic™ Dog HTR1A in Vitro [35S]GTPγS binding Assay	Dog	CHO-K1	[35S]GTPγS binding Assay
S01YF-1122-KX866	Magic™ Dog HTR1A in Vitro Radioligand Binding Assay	Dog	CHO-K1	Radioligand Binding Assay
S01YF-1122-KX871	Magic™ Mouse HTR1A in Vitro [35S]GTPγS binding Assay	Mouse	CHO-K1	[35S]GTPγS binding Assay
S01YF-1122-KX872	Magic™ Mouse HTR1A in Vitro Radioligand Binding Assay	Mouse	CHO-K1	Radioligand Binding Assay
S01YF-1122-KX873	Magic™ Rabbit HTR1A in Vitro [35S]GTPγS binding Assay	Rabbit	CHO-K1	[35S]GTPγS binding Assay
S01YF-1122-KX874	Magic™ Rabbit HTR1A in Vitro Radioligand Binding Assay	Rabbit	CHO-K1	Radioligand Binding Assay
S01YF-1122-KX875	Magic™ Rat HTR1A in Vitro [35S]GTPγS binding Assay	Rat	CHO-K1	[35S]GTPγS binding Assay
S01YF-1122-KX876	Magic™ Rat HTR1A in Vitro Radioligand Binding Assay	Rat	CHO-K1	Radioligand Binding Assay

Product Information

Target Protein

HTR1A

Target Family

5-Hydroxytryptamine Family

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

GPCR Cell Lines

Target Research Area

CNS Research

Related Diseases

Periodic Fever; Menstrual Cycle-Dependent; Generalized Anxiety Disorder

Gene ID

3350

UniProt ID

P08908

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The 5-hydroxytryptamine receptor 1A (HTR1A) gene codes for the 5-HT1A receptor. The neocortex, hypothalamus, and substantia gelatinosa of the spinal cord all contain a large number of 5-HT1a receptors and subtypes, which play a role in controlling hypothalamic mechanisms and cerebral cortex integrative processes. 5-HT1a receptors are particularly prevalent in the hippocampus, septum, amygdaloid nuclei, and dorsal Raphé nuclei, which are all implicated in the modulation of emotion. The fact that dorsal Raphé nuclei have 5-HT1a receptors shows that 5-HT can modify serotonergic neurons' activity. A flat body posture, reciprocal forepaw treading, and head weaving are signs that the central 5-HT1a receptors have been activated by an agonist. Traditional 5-HT1a receptor antagonists, as well as the -adrenergic receptor antagonists propranolol or pindolol, block these behavioral activations. This shows a connection between 5-HT1a receptors and β-adrenergic receptors in the central nervous system. By way of protein kinase A, chloride channels are made active when the 5-HT1a receptor is activated. The customized HTR1A stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Newman (Verified Customer)

What host cells are typically used? Nov 17 2022

chat Sherry Smith (Creative Biolabs Scientific Support)

HEK293 or CHO-K1 are used for this stable cell line. Nov 17 2022

chat Carl (Verified Customer)

What is the quality control for the HTR1A stable cell line? May 14 2023

chat Sherry Smith (Creative Biolabs Scientific Support)

This includes testing for bacterial and mycoplasma contamination, flow cytometry to verify the surface expression of HTR1A at the protein level, and qPCR to verify the engineering results at the mRNA level. May 14 2023

Published Data

Fig.1 Cre/tta-inducible and tomato-tagged allele of htr1a.

Extracts from CHO cells transfected with Htr1a-P2A-tdTomato or uncleavable Htr1a-*P2A-tdTomato plasmid showed the predicted cleaved P2A-tdTomato band and the uncleaved Htr1a-*P2A-tdTomato band, respectively, on a Western blot using anti-RFP or anti-Actin control antibodies.

Ref: Piszczek, Lukasz, et al. "Modulation of anxiety by cortical serotonin 1A receptors." Frontiers in behavioral neuroscience 9 (2015): 48.

Pubmed: 25759645

DOI: 10.3389/fnbeh.2015.00048

Research Highlights

The analysis's findings suggest that the HTR1A rs6295 polymorphism may affect the improvement of negative symptoms but not the improvement of either overall symptoms or positive symptoms.
Takekita, Yoshiteru, et al. "HTR1A polymorphisms and clinical efficacy of antipsychotic drug treatment in schizophrenia: a meta-analysis." International Journal of Neuropsychopharmacology 19.5 (2016): pyv125.
Pubmed: 26568455 DOI: 10.1093/ijnp/pyv125

These findings support the hypothesis that HTR1A and HTR5A play a role in the etiology of schizophrenia and raise the possibility that certain genetic variants in HTR5A contribute to executive dysfunction.
Guan, Fanglin, et al. "Evaluation of association of common variants in HTR1A and HTR5A with schizophrenia and executive function." Scientific reports 6.1 (2016): 38048.
Pubmed: 27897266 DOI: 10.1038/srep38048