mProX™ Human HLA-E Stable Cell Line

Product Information

Target Family

HLA

Target Protein Species

Human

Host Cell Type

HEK293;CHO-K1;SU.86.86

Target Classification

HLA Cell Lines

Target Research Area

Infectious Research

Related Diseases

Rabies; Choriocarcinoma

Gene ID

Human:3133

UniProt ID

Human:P13747

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

HLA-E, a non-classical major histocompatibility complex (MHC) molecule, has various applications in different fields. In the context of gestational diabetes mellitus (GDM), HLA-E is identified as one of the differentially expressed genes (DEGs) in placenta tissues of GDM patients. Its down-regulation suggests a potential role in GDM prevention and management. In Alzheimer's disease (AD), HLA-E is involved in the abnormal signal transduction of major histocompatibility complex class I (MHC-I) and is associated with increased content of GZMK+ CD8+ T cells in the peripheral blood. These GZMK+ CD8+ T cells, along with their interactions with other cell types, may serve as potential blood biomarkers of AD. In nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) and T-cell/histiocyte-rich large B-cell lymphoma (THRLBCL), the immune cell phenotypes accompanying different histological patterns involve expansions and depletions of specific immune cell subsets, including HLA-E-expressing cells. These findings provide insights into the immunoregulatory mechanisms and potential biomarkers of high-risk disease in NLPHL and THRLBCL. In the context of influenza virus infection, a cryptic MHC-E epitope derived from the influenza matrix gene segment elicits a potent cytolytic T cell response. This unconventional epitope, presented by MHC-E, highlights the importance of noncanonical translation products in the T cell repertoire and suggests therapeutic potential. Lastly, in human cytomegalovirus (HCMV) surveillance, HLA-E-restricted HCMV peptides are recognized by adaptive NK cells and induce memory responses. These unconventional peptides elicit aNK cell memory responses restricted to HCMV and provide insights into HLA-E-mediated signaling networks that mediate aNK cell recall responses. Overall, HLA-E has diverse applications in GDM, AD, lymphoma, viral infections, and immune cell memory responses.

Protocols

Please visit our protocols page.

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FAQ

chat Peyton Johnson (Verified Customer)

Does HLA-E play a role in immune response to infections? Nov 08 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

HLA-E is involved in the immune response, particularly in presenting antigens to T cells in the context of infections such as Epstein-Barr virus and Chlamydia trachomatis. Nov 08 2021

chat Skyler Garcia (Verified Customer)

How does HLA-E interact with other HLA molecules? Feb 23 2023

chat Patrick Liam (Creative Biolabs Scientific Support)

HLA-E can interact with other HLA class I molecules, influencing immune responses and potentially affecting disease outcomes, such as in HIV control. Feb 23 2023

Published Data

Fig.1 The evaluation of PDAC SU86.86 cell cytotoxicity in response to indicated treatments was conducted through LDH release assays at varying E:T ratios, with NK cells being employed.

The functional investigation of whether CTCs are evaded by NK cell surveillance through the HLA-E:CD94-NKG2A checkpoint was undertaken through an NK cytotoxicity assay. Protection from patient-derived NK cell killing at two effectors-to-target ratios by PDAC SU86.86 cells was achieved through the overexpression of HLA-E.

Ref: Liu, Xiaowei, et al. "Immune checkpoint HLA-E: CD94-NKG2A mediates evasion of circulating tumor cells from NK cell surveillance." Cancer Cell 41.2 (2023): 272-287.

Pubmed: 36706761

DOI: 10.1016/j.ccell.2023.01.001

Research Highlights

Duan, Tingting. et al. "Identification of Peripheral Blood GZMK+ CD8+ T Cells As Biomarkers of Alzheimer's Disease Based on Single-Cell Transcriptome." Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2023.
This research derives insights from single-cell RNA sequencing (scRNA-seq) to investigate the immune characteristics of patients with Alzheimer's disease (AD) in the peripheral blood, with the aim of identifying potential biomarkers. Through scRNA-seq, the genetic expression of individual cells can be analyzed, providing a deeper understanding of the immune response in AD. These findings may contribute to the development of early detection methods and potentially aid in the management of AD.
Duan, Tingting. et al. "Identification of Peripheral Blood GZMK+ CD8+ T Cells As Biomarkers of Alzheimer's Disease Based on Single-Cell Transcriptome." Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2023.
Pubmed: 37866940 DOI: 10.12182/20230960107

Panayi, Christos. et al. "Microenvironmental immune cell alterations across the spectrum of nodular lymphocyte predominant Hodgkin lymphoma and T-cell/histiocyte-rich large B-cell lymphoma." Frontiers in oncology, 2023.
The nodular lymphocyte predominant Hodgkin lymphoma (NLPHL), also referred to as nodular lymphocyte predominant B-cell lymphoma, shares some features with T-cell/histiocyte-rich large B-cell lymphoma (THRLCBL). The structure of NLPHL and the composition of its microenvironment are subject to variations, particularly in terms of B-cell and T-cell presence. Unfortunately, the immune cell phenotypes associated with different histological patterns are not well understood.
Panayi, Christos. et al. "Microenvironmental immune cell alterations across the spectrum of nodular lymphocyte predominant Hodgkin lymphoma and T-cell/histiocyte-rich large B-cell lymphoma." Frontiers in oncology, 2023.
Pubmed: 37854674 DOI: 10.3389/fonc.2023.1267604