mProX™ Human HCN4 Stable Cell Line

Product Information

Target Protein

HCN4

Target Family

Hyperpolarization-activated, Cyclic Nucleotide-gated Channels

Target Protein Species

Human

Host Cell Type

CHO-K1; HEK293

Target Classification

Ion Channel Cell Lines

Target Research Area

Cardiovascular Research; CNS Research

Related Diseases

Sick Sinus Syndrome; Brugada Syndrome

Gene ID

10021

UniProt ID

Q9Y3Q4

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

The hyperpolarization-activated funny current necessary for sinoatrial node automaticity is mediated by the HCN4 channels. This is demonstrated in transgenic mice that had the HCN4 gene specifically knocked out in the heart, resulting in severe progressive bradycardia. These channels are strongly expressed in the sinus node, and it has also been discovered that familial SND is linked to mutations in the HCN4 gene. These loss-of-function mutations cause a decrease in the diastolic depolarization range of sinoatrial cells, which can lead to a variety of abnormalities and decreased impulse generation. More variations with sinus bradycardia, chronotropic incompetence, and atrial fibrillation have been found in various families. The customized HCN4 stable cell line can be used in antibody discovery and development, potential drug candidate screening and signaling pathway researches.

Protocols

Please visit our protocols page.

Customer Reviews

chat Jennifer

I recently had the opportunity to try the HCN4 cell line from Creative Biolabs, and I am quite impressed. The product arrived promptly, and the packaging was secure. The provided instructions were clear and easy to follow. Apr 22 2020

chat Verified Customer

chat Jessica

I can't speak highly enough about the HCN4 cell line. It has exceeded my expectations in terms of performance and has become an invaluable asset in our lab. Sep 06 2023

chat Verified Customer

FAQ

Any questions about our products? Please visit our frequently asked questions page.

Published Data

Fig.1 HCN4 stably expressed in HEK293 cells.

HCN4 protein from a stable HCN4 cell line may be detected (right) but not HEK293 cell protein (left). Confocal photo of anti-HCN4 antibody-immunized HCN4 stable cell lines.

Ref: Ye, Bin, et al. "Caveolin-3 associates with and affects the function of hyperpolarization-activated cyclic nucleotide-gated channel 4." Biochemistry 47.47 (2008): 12312-12318.

Pubmed: 19238754

Research Highlights

The role of the HCN4 encoded hyperpolarization-activated 'funny' current (If) in pacemaker activity in the mammalian sinoatrial node (SAN), and the human SAN specifically, remains debatable and incompletely understood despite the numerous studies conducted over the previous forty years.
Hoekstra, Maaike, et al. "HCN4 current during human sinoatrial node-like action potentials." Progress in Biophysics and Molecular Biology 166 (2021): 105-118.
Pubmed: 34153331 DOI: 10.1016/j.pbiomolbio.2021.05.006

The sympathetic and parasympathetic nerve systems, which govern the sinoatrial node (SAN), dynamically regulate heart rate. The well-known causal molecule of congenital sick sinus syndrome is HCN4 pacemaker channels.
Kozasa, Yuko, et al. "HCN4 pacemaker channels attenuate the parasympathetic response and stabilize the spontaneous firing of the sinoatrial node." The Journal of physiology 596.5 (2018): 809-825.
Pubmed: 29315578 DOI: 10.1113/JP275303