mProX™ Human GRM7 Stable Cell Line

Product Information

Target Protein

GRM7

Target Family

Metabotropic Glutamate Family

Target Protein Species

Human

Host Cell Type

H460;CHO-K1;HEK293

Target Classification

GPCR Cell Lines

Target Research Area

CNS Research

Related Diseases

Neurodevelopmental Disorder With Seizures, Hypotonia, And Brain Imaging Abnormalities;Autosomal Recessive Non-Syndromic Intellectual Disability.

Gene ID

Human: 2917

UniProt ID

Human: Q14831

Product Properties

Biosafety Level

Level 1

Activity

Yes

Quantity

10⁶ cells per vial

Applications

GRM7 is associated with the metabotropic glutamate receptor 7 (mGluR7). This receptor has been implicated in various neurological functions and disorders. Recent studies have identified mutations in GRM7 that are associated with neurodevelopmental disorders. Specifically, these mutations can lead to reduced expression of mGluR7, which in turn can cause neurological dysfunction. Additionally, GRM7 variants have been explored in the context of their association with psychiatric conditions such as schizophrenia.

Protocols

Please visit our protocols page.

Customer Reviews

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FAQ

chat Dorothy (Verified Customer)

How is GRM7 associated with neurological disorders? Oct 07 2021

chat Patrick Liam (Creative Biolabs Scientific Support)

Mutations in GRM7 have been linked to developmental delay and neurological dysfunction. Specific mutations can reduce mGlu7 expression, leading to neurological phenotypes. Oct 07 2021

chat Amy (Verified Customer)

What role does GRM7 play in axon and presynaptic terminal development? Jul 16 2022

chat Patrick Liam (Creative Biolabs Scientific Support)

Pathogenic GRM7 mutations can impair axon outgrowth and the development of presynaptic terminals. Stable neuronal surface expression of mGLU7 is essential for these neurological processes. Jul 16 2022

Published Data

Fig.1 n H460-TL cells, we conducted a tumor sphere formation assay comparing shControl with silenced DRD2, HRH3, and GRIN2B genes, alongside Control and cells overexpressing GRM7.

A tumor sphere formation assay was conducted using H460-TL cells in various conditions, including shControl, DRD2-silenced, HRH3-silenced, GRIN2B-silenced, Control, and GRM7-overexpressing cells, each seeded with 400 cells per well, across three independent experiments. The blue columns represent H460 cells with shControl or Control conditions, while various types of red columns represent cells with silenced or overexpressed genes. Representative images of the tumor spheres were captured, and the data is presented as mean ± s.e.m. values. Statistical analysis involved one-way ANOVA with uncorrected Fisher's LSD test for knockdown cell lines and unpaired two-tailed Student's t-test with Welch's correction for GRM7-overexpressing cells, with significance denoted as *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, or N.S. for non-significant results.

Ref: She, Xiaofei, et al. "A high-throughput screen identifies inhibitors of lung cancer stem cells." Biomedicine & Pharmacotherapy 140 (2021): 111748.

Pubmed: 34044271

DOI: 10.1016/j.biopha.2021.111748

Research Highlights

Liu J, et al. "A pilot study on glutamate receptor and carrier gene variants and risk of ." Metabolic brain disease, 2023.
In the study, it was found that the genotypes and allele distributions of certain SNPs were not significantly associated with the risk of ASD. However, two specific SNPs, rs1800656 and rs2237731 in the GRM8 gene, were found to be linked to the severity of language impairment. No other SNPs were found to be related to the overall severity of ASD. The results suggest that there is an association between the SLC25A12 gene variant and the risk of childhood ASD, as well as between the GRM8 gene variant and the severity of language impairment in the Chinese Han population. This study was conducted on a population of Chinese Han individuals and the data was analyzed using statistical methods (1.1-2.6, P = 0.0107).
Pubmed: 37578654 DOI: 10.1007/s11011-023-01272-w

Creighton CJ, et al. "Comparative and integrative analysis of transcriptomic and epigenomic-wide DNA ." Epigenetics, 2023.
The study identified top candidate genes with significant hypermethylation and down-regulated gene expression in hemidesmosome assembly, mammary gland development, epidermis development, hormone biosynthesis, and cell communication. Similarly, significant hypomethylation and up-regulated gene expression were observed in macrophage differentiation, cAMP-dependent protein kinase activity, protein destabilization, transcription co-repression, and fatty acid biosynthesis. Contrasting findings were also observed, with the AA dataset showing enrichment of genes involved in steroid signaling, immune signaling, chromatin structure remodeling, and RNA processing compared to the TCGA dataset. AMIGO3, IER3, UPB1, GRM7, TFAP2C, TOX2, PLSCR2, ZNF292, ESR2, MIXL1, BOLL, and FGF6 were identified as significant and uniquely associated with PCa progression in the AA cohort.
Pubmed: 37279148 DOI: 10.1080/15592294.2023.2180585